used for analysis by HPLC-DAD-ED Time (min) Eluent
A (%) Eluent
B (%) 0.10 98.90 1.10 15 91.00 9.00 20 87.80 12.20 30 87.80 12.20 55 86.50 13.50 95 73.00 27.00 105 37.00 63.00 110 37.00 63.00 125 98.90 1.10 130 98.90 1.10 HPLC: High performance liquid chromatography, DAD: Diode array detection, ED: Electrochemical detector Table 2.
The 'IC SI-36 4D' column for ion chromatography analyzes anions, and is compatible with hydroxide eluents
. SDK will place the 'IC SI-36 4D' on the market by the end of the year.
Gradient elution involves multiple eluents
(aqueous and organic, polar and non-polar) that are mixed in different ratios by a program in the machine (Figure 1).
Acid (acetic or formic) to obtain appropriate pH (3-5.5) and salt (ammomium acetate or ammonium formate) at the concentration range 5-20 mM were added to tested eluents
because blocking and suppressing the ionization of residual silanol groups of the stationary phase were necessary to avoid peak splitting or broadening of basic compounds.
Two LC Column Poroshell 120 EC-C18 3 x 50 mm, 2.7 [micro]m, were used to eluent
A 100 mL of each eluent
was added to 0.1 g of loaded biosorbent, this mixture was agitated in a temperature controlled water bath shaker operated at 26 o C and 150 rpm for 120 minutes.
The last eluent
was crucial in obtaining purified hGDNT.
So, we just collected directly and combined the eluents
in the tubes within the same peak and dismissed others.
The particles were ultimately separated, rinsed with methanol dichloromethane solution (v/v, 4 : 1) for 5 times, and extracted with 0.3 mol/L sodium hydroxide methanol as eluent
using the Soxhlet method, until no template molecule TBT contained the polymer.
For the experiments with ACN, the solution of the first eluent
was prepared by dissolving 19.0 g sodium acetate trihydrate in 1 L Milli-Q water, adding 0.5 mL TEA, and adjusting the solution to pH 6.40 with glacial acetic acid.
Antocyanins were sorbed from the concentrate onto the cartridge sorbent, the cartridge was washed with 1ml of HC1, and anthocyanins were re-extracted by the eluent
containing 30 vol.
EDTA-containing blood samples and DBS eluents
were spiked with recombinant human FXN81-210 to a final concentration of 200 ng/mL and serially diluted to investigate recovery, linearity, limit of detection, and functional sensitivity.