The reason for this difference in the behavior of the model pharmaceuticals when varying ACN content in the
eluent is due to the hydrophilicity of pharmaceuticals, as it is clear from the log Pow values of the pharmaceuticals.
1) GPC (gel permeation chromatography): A variety of SEC, uses organic solvents (such as tetrahydrofuran and chloroform) as
eluent.
The retention times for the two epimers on all four columns were found to be similar, and were unaffected by the pH of the
eluent.
The desorption efficiency of the
eluents used was found to be generally low, it follows the order HClgreater than CH COOHgreater than H O with corresponding values of 17.
The purpose of the CP analyte was to serve as an internal control for measuring FXN, and thus a common
eluent volume was selected to best match the linear response of our anti-FXN antibody pair.
Eluent A was water and
eluent B was acetonitrile--water (95:5), both containing 2 mM ammonium formate and 50 mM formic acid (Quilliam 2003).
The solvent was removed under reduced pressure and the residue was purified by column chromatography through silica gel using as an
eluent a mixture of n-hexane and ethyl acetate (9:1) to give 7 (1.
1mm, column particle size= 7[micro]m, stable pH=0-14, Hamilton, USA) with 6mM NaOH
eluent was used (flow rate= 1ml/min).
The column was eluted with chloroform-methanol (95:5) and the
eluent collected in volumes of 2.
The
eluent was collected on a fraction collector in volumes of 3.
Retention time varies depending on the interactions between the stationary phase, the molecules being analyzed, and the
eluent used with a conductivity or UV-Vis detector.
Eluent from hands and PPE was assayed by the most probable number (MPN) enrichment infectivity assay (8).
