5 mol/L HCl solution as an
eluant indicating that the resin can be regenerated and reused.
Endonuclease activity was determined by incubating 1 [mu]g plasmid (pVAXl, Invitrogen) with 19 [mu]1 of
eluant fractions from the protein purification columns (DEAE-52 or Sephadex G-75) at 37 [degrees]C for 30 min.
Eluant was directed into an electrospray mass spectrometer (API 365; Applied Biosystems, Foster City, CA).
The
eluant was tetrahydrofuran flowing at 1 mL/min and at temperature of 35[degrees]C.
In two separate recovery experiments employing commercial standards, the first 10 mL of
eluant (including the 5-mL void volume) contained 88% of the proline and the second 15 mL of
eluant contained 97% of the ornithine standard.
This means an entirely new
eluant system must be developed that is completely compatible with the mass spectrometry.
IEC, effect of
eluant concentration, and analysis of elution behavior were also carried out to understand the IECs of the AECFs.
Despite apparent bead loss into the
eluant during the 5th run (suggesting column failure), the depletion efficiency was 99% for that run.
It allows the user to determine perchlorate levels in drinking water down to 1 ppb using a simple carbonate/bicarbonate
eluant and suppressed conductivity detection.
One major carbohydrate peak was detected in the void volume of the S-200 column when the 4% NaOH extract was chromatographed with 7 M urea as
eluant (Fig.
The analytical equipment consisted of an HPLC DX-300 series (Dionex, Sunnyvale, CA) with a quaternary gradient pump system, an
eluant degas module, an autosampler with cooling module model AS 3500 (Dionex, Sunnyvale, CA) fitted with a 25-[micro]L injection loop.
4]) and concentrated to give a thick liquid which on silica gel chromatography using hexane as the
eluant gave tetraallyl compound 4 as a colourless liquid (21 g, 47% based on the amount of diamine I present after reaction with ethyl formate).
