electroporation


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e·lec·tro·por·a·tion

(ē-lek'trō-pōr-ā'shŭn),
A technique in which a brief electric shock is applied to cells; momentary holes open briefly in the plasma membrane, allowing the entry of macromolecules (for example, a way of introducing new DNA into a cell).

electroporation

(ĭ-lĕk′trō-pə-rā′shən)
n.
A process of applying a high-voltage electrical pulse to a living cell, causing temporary permeability of the cell membrane, through which a foreign material such as DNA may pass.

electroporation

a method in which CELLS are subjected to an electrical impulse that leads to the temporary formation of pores in the cell MEMBRANE. Such pores enable commodities such as DNA, PROTEINS and ATP to pass into the cell, before the pores are repaired. Electroporation provides a means of transforming cells (see TRANSFORMATION). It can also be used to deliver DRUGS across the skin and to transfer GENETIC MATERIAL to targetted cells, in GENE THERAPY.
References in periodicals archive ?
Draghia-Akli, "Electroporation for the delivery of DNA-based vaccines and immunotherapeutics: current clinical developments," Molecular Therapy, vol.
In molecular cloning, Plasmid transformation into the bacterial competent cells by using electroporation has been found an efficient technique (Ryu and Hartin, 1990).
Pre-experimental tests were conducted due to differences in hardware, and settings were fine-tuned until tuber ablation size was consistently well within the margins of the tuber, but also within parameters commonly used in clinical electroporation. Target amperage was 20-30 amp, similar to clinical IRE ablation.
Electroporation instruments allow for efficient transfection by inducing transient pores to form in the cell membrane in response to a carefully controlled electrical pulse.
tumefaciens transformation was done by electroporation method as mentioned previously (Yanagisawa et al., 2004).
In such way magnetoporation allows contactless cell membrane permeabilization without side effects of electroporation [14], [27]-[29].
The reversible or irreversible electroporation can lead to different treatment outcomes.
Such techniques consist of utilizing the chemical solvent, iontophoresis, electroporation, sonophoretic, microneedle application, skin shrinkage, heating and prodrug [2].
Available Samples: Mirus/MoBiTec consider sample requests for up to three different transfection products and one electroporation product per laboratory.
FireflySci's model of electroporation cuvettes calledType 704P are strictly manufactured to efficiently transfer DNA and RNA into a wide variety of cells.
After electroporation (12.5 kV/cm, 20 ms), the samples were immediately plated using two plating methods: direct plating or overlay cultivation.
In previous studies, we could indeed corroborate that the incorporation of the IL-15 transpresentation mechanism into currently used DC vaccines by means of mRNA electroporation increases their immunostimulatory properties towards both NK cells [16] and [CD8.sup.+] T-cells [17].