electroporation

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e·lec·tro·por·a·tion

(ē-lek'trō-pōr-ā'shŭn),
A technique in which a brief electric shock is applied to cells; momentary holes open briefly in the plasma membrane, allowing the entry of macromolecules (for example, a way of introducing new DNA into a cell).

electroporation

(ĭ-lĕk′trō-pə-rā′shən)
n.
A process of applying a high-voltage electrical pulse to a living cell, causing temporary permeability of the cell membrane, through which a foreign material such as DNA may pass.

electroporation

[-pôrā′shən]
a type of osmotic transfection in which an electric current is used to produce temporary holes in cell membranes, allowing the entry of nucleic acids or macromolecules (a way of introducing new deoxyribonucleic acid into the cell). See also transfection.

electroporation

a method in which CELLS are subjected to an electrical impulse that leads to the temporary formation of pores in the cell MEMBRANE. Such pores enable commodities such as DNA, PROTEINS and ATP to pass into the cell, before the pores are repaired. Electroporation provides a means of transforming cells (see TRANSFORMATION). It can also be used to deliver DRUGS across the skin and to transfer GENETIC MATERIAL to targetted cells, in GENE THERAPY.

electroporation (i·lekˈ·trō·p·rāˑ·shn),

n technique by which cell membranes are made permeable by rapid pulses of high-voltage current. Has been used to treat cancers.

electroporation

the use of high-voltage electrical impulse to create pores through a cell membrane and allow uptake of DNA into a cell.
References in periodicals archive ?
subtilis 168, screening results showed that electroporated cells can grow in supplemented media containing neomycin antibiotic.
These PCR products were sequentially ligated into the p2NIL plasmid, then the PacI cassette from the pGOAL19 plasmid was ligated into the resulting recombinant plasmid and the final suicide plasmid obtained was electroporated into M.
LC-MS/MS revealed that DBP and DEHP entered the cells, where they were converted to monoesters, whereas the monoesters MBP and MEHP were excluded probably because of their negative charge (Figure 2F)-Because MBP has been argued to be the active metabolite of DBP, we electroporated 10 [micro]M and 100 [micro]M MBP into the SC5 cells, which at 100 [micro]M resulted in a significant inhibition of PGD2 after 6 hr (Figure 2G).
The plasmid DNA was then electroporated into competent Saccharomyces cerevisiae cells.
The buffer solution containing one million protoplasts/ml, mixed with plasmid DNA pBIN35S-mGFP4, were electroporated at 375 V/cm.
This apparatus includes a continuous flow shunt allowing blood to be withdrawn from the patient and a pre-sterilized, disposable chamber in which the cells in this blood can be electroporated, allowing therapeutic genes or drugs to be infused into the cells.
AGS-004 is produced from autologous, monocyte-derived dendritic cells that are electroporated with RNA encoding for CD40L and for HIV antigens Gag, Nef, Rev, and Vpr, derived from each patient's pre-ART plasma.
For transfection of R1 cells, bovine wild type (-134/+84) and Sp1 mutant Nanog promoter-green fluorescence protein (GFP) reporter vectors and pRL-TK, a transfection control vector, were linearized, and were electroporated into 1 x [10.
About 5 g of pET-32a-IERS- EGFP plasmid was respectively electroporated into MDBK-T7 and MDBK-negative using a Bio-Rad Gene Pulser Xcell (Gene Pulser(R) Electroporation Buffer, 340 V, 5 ms, 4 mm cuvettes, one pulse).
Two plant expression vectors, pGNC1-Rep and pGN35S were electroporated into Agrobacterium tumefaciens strain GV3101 (Fiedle and Wirth, 1988).
About 20 [mu]g plasmid DNA was added to the cells and electroporated with the square wave program with three 350 V pulses using a Bio Rad Gene Pulser.