electrophoresis


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electrophoresis

 [e-lek″tro-fo-re´sis]
the movement of charged particles suspended in a liquid on various media (e.g., paper, gel, liquid) under the influence of an applied electric field. adj., adj electrophoret´ic. The various charged particles of a particular substance migrate in a definite and characteristic direction—toward either the anode or the cathode—and at a characteristic speed. This principle has been widely used in the separation of proteins and is therefore valuable in the study of diseases in which the serum and plasma proteins are altered. The principle also has been applied in the separation and identification of various types of human hemoglobin.

e·lec·tro·pho·re·sis

(ē-lek'trō-fōr'ē-sis),
The movement of particles in an electric field toward an electric pole (anode or cathode); used to separate and purify biomolecules.
See also: electropherogram.
[electro- + G. phorēsis, a carrying]

electrophoresis

(ĭ-lĕk′trō-fə-rē′sĭs)
n.
1. The migration of charged colloidal particles or molecules through a stationary medium under the influence of an applied electric field usually provided by immersed electrodes. Also called cataphoresis.
2. A method of separating substances, especially proteins, and analyzing molecular structure based on the rate of movement of each component in a colloidal suspension while under the influence of an electric field.

e·lec′tro·pho·ret′ic (-rĕt′ĭk) adj.

electrophoresis

Lab methods A method of separating large molecules–eg, DNA fragments or proteins from a mixture of similar molecules, by passing an electric current through a medium containing the mixture; each molecule travels through the medium at a different rate, depending on its electrical charge and size; agarose and acrylamide gels are media commonly used electrophoretic media

e·lec·tro·pho·re·sis

(ĕ-lek'trō-fŏr-ē'sis)
The movement of particles in an electric field toward anode or cathode.
See also: electropherogram
Synonym(s): ionophoresis, phoresis (1) .

electrophoresis

Separation of charged particles in a solution (ions) by the application of an electric current. This can be done in a thin layer of solution on paper or in a gel. Ions of low weight move more quickly than those of high weight, so separation occurs and can be demonstrated by staining. The method is widely used in medicine to identify and measure the proteins present in the blood including the ANTIBODIES (IMMUNOGLOBULINS). It is used to identify the various abnormal haemoglobins causing SICKLE CELL ANAEMIA and other similar conditions. It is extensively used in genetic work such as DNA fingerprinting. Electrophoresis is remarkably sensitive. Pieces of DNA, for instance, that differ in length from each other by only one base pair can be separated into discrete bands by this method.

electrophoresis

a method for separating particles with different electrical charges, for example, amino acids, peptides, proteins and nucleic acids. The apparatus consists of a supporting medium soaked in a suitable buffer with an electrical field set up across it. The mixture to be separated (e.g. blood proteins) is placed on the supporting medium. The components with different charges then separate from each other and their eventual position is compared with the position of known standards.

Electrophoresis

Use of an electrical field to separate proteins in a mixture (such as blood or urine), on the basis of the size and electrical charge of the proteins.

e·lec·tro·pho·re·sis

(ĕ-lek'trō-fŏr-ē'sis)
The movement of particles in an electric field toward anode or cathode.
Synonym(s): ionophoresis, phoresis (1) .
References in periodicals archive ?
Lunte, "Enhanced pH-mediated stacking of anions for CE incorporating a dynamic pH junction," Electrophoresis, vol.
Comparison of systems used for identification of hemoglobin variants including the Primus Ultra2 High Performance Liquid Chromatography (HPLC) System and The Sebia CAPILLARYS 2 Capillary Electrophoresis (CE) Systems.
Simulation of gel electrophoresis using DNA samples cut from paper for paternity testing (see reference).
* A1: there are no sickle-cell screening services at district health centres and regional hospitals, and all children are referred to Mulago Hospital for haemoglobin electrophoresis
Bio-Rad offers the Experion automated electrophoresis system.
All Finnish and French isolates showed a high similarity with a minimum of 80.3% overall relatedness by using pulsed-field gel electrophoresis (PFGE) analysis after digestion of B.
Automated machines today decipher the genomes of people and other organisms by carrying out electrophoresis of DNA pieces within fine glass tubes (SN:8/6/05 p.85).
The new system, which fully automates all processes from isoelectric focusing to polyacrylamide gel electrophoresis to detection, can perform two-dimensional electrophoresis of proteins in about one hour.
To address this issue, capillary electrophoresis using an ultraviolet-visible photodiode array detector was employed as an alternative analytical tool.
An Electric Focusing System which allows scientists and researchers to conduct a wide range of flat bed electrophoresis applications running up to 1500 volts, is the latest addition to the SCIE-PLAS range of electrophoresis products.

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