For example, Aquadro and Patton (1980) demonstrated that species-specific differences in salivary amylase electromorph
phenotypes could be used to unambiguously distinguish between morphologically similar Peromyscus leucopus and P.
Isolates for which 1 signal was observed for a given locus on the electromorph
were considered homozygous for this locus by analogy with what is reported for another diploid yeast, C.
Efficiency of SSCP procedures to identify sequence variants was assessed by sequencing multiple representatives of each electromorph
and comparing patterns of sequence divergence among them.
Interpreting stained electromorph
bands on a gel requires a thorough understanding of the genetic basis of allelic variation for each enzyme (reviewed by Richardson et al.
was considered to represent a distinct allele of the same enzyme.
The fast electromorph
of AAT was only observed in the Duke Forest populations, whereas alleles of GPI and EST were widespread and polymorphic within most populations.
At these loci, the plant samples from each population were homozygous for a single electromorph
Upon completion of the initial survey, samples of each variant electromorph
were rerun in lineup gels to assign relative mobility and establish the veracity of the initial interpretation.
With the exception of one or two bands attributable to molecular heterozygosity in dimeric enzymes, each electromorph
was treated for purposes of analysis as a single allele at one genetic locus.
cornutus exhibits this fast electromorph
and, more commonly, a unique slow allele (Dowling and Moore 1984).
The frequencies of the Pgm-3 alleles [TABULAR DATA FOR TABLE 1 OMITTED] found in each sample in this study and the relative electrophoretic mobility of each allelic electromorph
are reported in the Appendix.
A slow electromorph
presumably representing GPI-[A.