Benzoic acid is a relatively common compound, so the researchers plan to look for a simple method to sense ecgonine
cocaine, benzoylecgonine, ecgonine
methyl ester in human urine by GC/MS;
 Nonstandard abbreviations: PS-MS, paper spray mass spectrometry; BZE, benzoylecgonine; EME, ecgonine
methyl ester; ACN, acetonitrile; PS, paper spray; A/IS, analyte-tointernalstandard ratio.
Saliva/plasma ratios of cocaine, ecgonine
methyl ester and benzoylecgonine following controlled cocaine administration.
Sweat patch specimens were analyzed for COC, BE, EME, ecgonine ethyl ester, cocaethylene, norcocaine, norcocaethylene, m- and p-hydroxycocaine, and m- and p-hydroxybenzoylecgonine by modifying the method published by Huestis et al.
The method limits of quantification (LOQ) were 2.5 ng/patch for COC, BE, EME, ecgonine ethyl ester, and cocaethylene and 5.0 ng/patch for all other analytes.
Benzoylecgonine (BE) and ecgonine
methyl ester (EME), major metabolites of COC, have longer half-lives in plasma and oral fluid than does the parent drug (5,9,11-14).
N-desmethyl-N-[[sup.2.H.sub.3]]methylcocaine ([d.sub.3] COC), BZ, N-des-methyl-N-[[sup.2.H.sub.3]]methylbenzoylecgonine ([d.sub.3]-BZ), N-desmethyl-N-[[sup.2.H.sub.3]]methylecgonine ([d.sub.3]-EC), ecgonine
, MED (anhydroecgonine methyl ester), and ED (anhydroecgonine) were purchased from Radian International.
Cocaine, BE, and ecgonine
methyl ester were isolated using solid-phase extraction.
The drug menu by class of substances included amphetamines, opiates (morphine-related compounds), methadone, cocaine, and cannabinoids, whereas the drug menu for identification/ quantification consisted of amphetamine, methamphetamine, morphine, codeine, 6-acetylmorphine, methadone, 1,5-dimethyl-3,3-diphenyl-2-ethylidene- pyrrolidine (EDDP), benzoylecgonine, ecgonine
methyl ester, and 11-nor-9-carboxy-[[DELTA].sup.9]-tetrahydrocannabinol.
This approach would appear to be a less cumbersome way to increase the positive rate for COC than a recent method that analyzes for ecgonine
Solid-phase extraction and GC/MS quantitation of cocaine, ecgonine
methyl ester, benzoylecgonine, and cocaethylene from meconium, whole blood, and plasma.