So far, two EAG isoforms Eag1 (KCNH1, Kv10.1) and Eag2 (KCNH5, Kv10.2) have been identified and they show similar electrophysiological features such as slow voltage-dependent activation, noninactivating, and inhibition by intracellular [Ca.sup.2+] [[[Ca.sup.2+]].sub.i] [4, 6, 7].
In physiological conditions, both EAG1 and EAG2 channels are expressed in the brain and their distributions overlap in the cortex and olfactory bulb, but show some differential expression pattern in other specific locations such as thalamus .
Besides EAG1, AA also activates EAG2 and ERG1 channels, implicating a conserved mechanism involved in AA-mediated activation of the EAG channel family [32, 80].
A recent report has shown that Arg327 mutation in EAG2 channel promotes the channel constitutively opening by reducing the interaction with other negatively charged residues .
Wray, "Molecular identification and characterisation of the human eag2 potassium channel," FEBS Letters, vol.
Heinemann, "Functional distinction of human EAG1 and EAG2 potassium channels," FEBS Letters, vol.
Karschin et al., "Cloning and functional expression of rat eag2, a new member ofthe ether-a-go-go family of potassium channels and comparison of its distribution with that of eag1," Molecular and Cellular Neurosciences, vol.
The metal oxide is zinc oxide; the covering layer is a sacrificial metal; and the thicknesses (eAg1, eAg2
, eAg3) of the silver layers (Ag1, Ag2, Ag3) vary in a ratio of between 0.8 and 1.2 and are such that eAg1 [less than or equal to] eAg3 [less than or equal to] eAg2
and eAg1 corresponds to the thickness of the silver layer closest to the substrate and eAg3 corresponds to the thickness of the silver layer furthest from the substrate.