References in periodicals archive ?
The size profiles represent the frequency distribution of cfDNA fragments at different lengths in bp for dsDNA (blue and red) and ssDNA (green and orange) library preparation.
Signal DNA can bind on the surface of SCGS due to the addition of target HIV-1 dsDNA. GOx can also bind to signal DNA by streptavidin-biotin bond after addition of streptavidin and biotin-GOx.
where [phi] is the helical phase angle parameter, R=10 [Angstrom] is the radius of the dsDNA helix, c = 34 [Angstrom] is the unit cell length of the dsDNA helix, and the parametric variables [THETA] and t are such that -[pi] < [THETA] < [pi] and -1 < t < 1.
The proposed algorithm has the benefit of fitting seamlessly into our established testing procedures while retaining the safety of not excluding patients from ENA and dsDNA antibody testing based upon DFS70 antibody testing outcomes.
Our results suggest that frequency in the arthralgia patient group was similar to previous studies (12/39), while patients with hematuria showed a moderately higher frequency (24/59).[sup] Intriguingly, one serum sample from an arthralgia child simultaneously showed responses to the SmD1[sub]83-119 peptide, the Sm antigen, dsDNA, and nucleosomes.
Role of lupus auto-antibodies (antinuclear antibody and dsDNA) and complements (C3/C4)
The kit adapts the company's patented SMART (Switching Mechanism at 5' End of RNA Template) technology in an innovative way for use with low-input samples, including both dsDNA and ssDNA templates, to generate ChIP-seq libraries for next-generation sequencing (NGS).
Instead, in vivo DNA replication methods for most organisms rely on a class of enzymes known as DNA helicases, which act to locally unwind dsDNA to create small ssDNA regions suitable for polymerase activity.
As one of the ACR SLE diagnostic criteria , IgG ANAs target a variety of nuclear antigens such as double-stranded DNA (dsDNA), which are intimately involved in SLE pathogenesis.
2011), and total stock of double-stranded DNA (dsDNA) (Sandaa et al.
Enzyme Linked Immunosorbent Assay technique was used for the detection and quantification of autoantibodies like ANA, dsDNA, anti-SSA, anti-SSB, anti-histone, and anti-Sm.
Immunological Parameters (ANA, dsDNA, Anti-Sm, Anti-SSAo, La, nDNA, Anti-histone) were measured by using Indirect ELISA Technique (Orgentec kits).
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