Su, "Micropatterned paper devices using amine-terminated polydiacetylene vesicles as colorimetric probes for enhanced detection of double-stranded DNA
," Sensors and Actuators B: Chemical, vol.
But large double-stranded DNA
viruses like poxviruses and herpesviruses are much slower to evolve than RNA viruses, leaving scientists wondering how they adapt to compete in the arms race with the immune system.
Incubation of splenocytes with 3 [micro]M mitomycin C for 1 and 4.5 h resulted in 23 and 32%, respectively, less double-stranded DNA
than in saline-treated cells (Table 2).
That method, created in the mid-1970s by Frederick Sanger of the Medical Research Council in Cambridge, England, starts with the isolation of long strings of double-stranded DNA
According to Stanford Medical School, "The picture of RNA polymerase at work provided an atomic-level window into how the protein complex unzips and then re-zips the double-stranded DNA
like a Ziploc bag after using the internal code to build a specific RNA molecule.
The researchers found a way to control the assembly of gold nanoparticles using rigid, double-stranded DNA
. Their technique takes advantage of this molecule's natural tendency to pair up components called bases, known by the code letters A, T, G and C.
The life of phages is discussed in contributions on DNA packaging in double-stranded DNA
phages, general aspects of lysogeny, gene regulatory circuitry of phage lambda, regulation of lambda gene expression by transcription termination and antitermination, and phage lysis.
Topo II, a nuclear protein that is essential for normal cellular processes such as replication, binds to double-stranded DNA
cutting it to relieve supercoiling.
Chlordecone also raised levels of antibodies to native, double-stranded DNA
, another specific marker for SLE.
These ribozymes were synthesized and used as templates in PCR reactions to generate double-stranded DNA
. Each was cloned into pPCR-Script by blunt-end ligation and transformed into XL-10 Gold Ultracompetent cells.
DNA damage can then be quantified by measuring the ratio of single- to double-stranded DNA
The small globular lesions, which look like M&M's attached to the skin, are caused by a large double-stranded DNA
virus of the poxvirus family.