HDF1 and RAD17 genes are involved in DNA double-strand break
repair in stationary phase Saccharomyces cerevisiae.
Right now, it appears that the most troubling side effects are observed when CRISPR tools cut both strands of DNA (a "double-strand break
The Cas9 nuclease in complex with crRNA-tracrRNA duplex induces the double-strand break
within the target DNA that is a prerequisite for genome editing [16, 40] (Figure 2).
Lobrich, "Evidence for a lack of DNA double-strand break
repair in human cells exposed to very low x-ray doses," Proceedings of the National Academy of Sciences of the United States of America, vol.
Jackson, "Mammalian DNA double-strand break
repair protein XRCC4 interacts with DNA ligase IV"' Current Biology, vol.
Chen, "DNA double-strand break
repair via non-homologous end-joining," Translational Cancer Research, vol.
Barton et al., "DNA Double-Strand Break
Resection Occurs during Non-homologous End Joining in G1 but Is Distinct from Resection during Homologous Recombination," Molecular Cell, vol.
Regulation of DNA double-strand break
repair pathway choice.
It could also inhibit recruitment of double-strand break
resection factors (i.e., RPA, Brca1, and Rad51) to DNA damage sites, leading to compromised homologous recombination repair.
On the other hand, BRCA1 interacts with RAD51 recombinase (RAD51) during homologous recombination mediated DNA double-strand break
repair forming a nucleoprotein filament on DNA and its mutation can increase the risk of breast cancer (9-11).
DNA double-strand break
(DSB) is considered the most critical type of DNA damage, inducible by ionizing radiation (IR) or a subset of drugs used in cancer chemotherapy.
He presents an overview of various double-strand break
(DSB) repair mechanisms, and discusses single-strand annealing, mitotic gene conversion, and break-induced replication.