At present, research on the mechanism of neuropathic pain has mainly focused on the peripheral mechanism and the central mechanism, with the central mechanism expressed as changes in synaptic plasticity in the spinal dorsal horn
, that is, central sensitization.
Consistent with previous studies, we found that spinal TSPO was upregulated in the dorsal horn
[GABA.sub.B] receptors are G protein-coupled metabotropic receptors which are widely distributed in the central nervous system, especially in the spinal cord dorsal horn
Following the manufacturer's protocol, total RNA was isolated from homogenized ipsilateral L4-L6 spinal dorsal horns
using the TRIzol reagent (Invitrogen Corp., Carlsbad, CA, USA).
(24, 26) We have shown that BDNF may cause downregulation of the chloride transporter KCC2 in a subpopulation of lamina I secondary sensory neurons in dorsal horn
, which then causes a depolarizing shift in the anion reversal potential ([E.sub.anion]).
Todd, "Neuronal circuitry for pain processing in the dorsal horn
," Nature Reviews Neuroscience, vol.
Glial Cell Profiling in the Dorsal Horn
of Rats with 8 Weeks of Experimental Diabetes.
Carstens, "Role of TNF-alpha in sensitization of nociceptive dorsal horn
neurons induced by application of nucleus pulposus to L5 dorsal root ganglion in rats," Pain, vol.
Roberts, "Anterior pretectal nucleus facilitation of superficial dorsal horn
neurones and modulation of deafferentation pain in the rat.," The Journal of Physiology, vol.
Henry, "Mediation and modulation by eicosanoids of responses of spinal dorsal horn
neurons to glutamate and substance P receptor agonists: Results with indomethacin in the rat in vivo," Neuroscience, vol.
SNI induced a robust astrocytic activation, indicated by GFAP upregulation in the ipsilateral spinal dorsal horn
of SNI group (Fig.