In this investigation, some oleic acid esters are synthesized using dodecyl
alcohol, propylene glycol and pentaerythritol as the alcoholic compartments.
The labelled ML I isoforms were analyzed by sodium dodecyl
sulfate-polyacrylamide gel electrophoresis (SDS-PAGE).
Comparison of lipopolysaccharide (LPS) antigens from the 2 strains by sodium dodecyl
sulfate--polyacrylamide gel electrophoresis and immunoblotting showed that they differed in O-repeating units (Figure, panel B).
Approximately 10-30 lag of protein was separated using electrophoresis in a 10% sodium dodecyl
10:15 USING SPINNING DROP TENSIOMETRY TO DETERMINE THE SQUARE GRADIENT PARAMETER FOR DODECYL
ACRYLATE) FOR USE IN THE TIPMPS FLIGHT INVESTIGATION
A single protein band was observed on a sodium dodecyl
sulfate polyacrylamide gel following purification under denaturing conditions and dialysis to remove the urea.
This enhanced fluorescence was obliterated by the addition of dodecyl
sulfate, suggesting that it was due to SULT1A1:DHC:PAP ternary complex.
3-[(3-Cholamidopropyl)-dimethylammonio]-1-propanesulphonate (CHAPS) and 3-[(3-cholamidopropyl)-dimethylammonio]-2-hydroxy-1-propanesulphonate (CHAPSO) were from Calbiochem (Darmstadt, Germany); N-(2-hydroxyethyl)-piperazine-N'-(2-ethanesulphonic acid) (Hepes), tris(hydroxymethyl)aminomethane (Tris), ethylene glycol-bis([beta]-aminoethyl ether)-N,N,N',N'-tetraacetic acid (EGTA), 1,6-diphenyl-1,3,5-hexatriene (DPH), sodium dodecyl
sulphate (SDS), phenylmethylsulphonyl fluoride (PMSF), aprotinin, benzamidine, digitonin, cholic acid, deoxycholic acid, n-dodecyl-[beta]-D-maltoside (DBM), and dodecylpoly[(ethylene-glycolether).sub.n] (Lubrol PX) were from Sigma Chemical Co.
Usuda and Shimogawara (1994) also showed, using sodium dodecyl
sulfate polyacrylamide gel electrophoresis (SDS-PAGE), that PEPCase and Rubisco protein content decreased gradually as thawing time increased.
The antioxidants to be studied are BHA, BHT, propyl gallate and dodecyl
gallate, and the initial phase of the project will involve the analysis of foods that have been commercially produced to determine the levels of recovery of antioxidant using different extraction techniques.
Sulfate, SDS, is a well known anionic surfactant
Passage in reverse order over dodecyl
agarose removes nearly all of the remaining protein, but the FRED elutes almost unretarded.