dl-glyceraldehyde

dl-glyceraldehyde

(glĭs″ĕr-ăl′dĕ-hīd)
An aldose, CHOCH(OH)CH2OH, produced by the metabolism of fructose in the liver.
References in periodicals archive ?
Phenylmethylsulfonyl fluoride (PMSF), DL-glyceraldehyde, and all other materials used were analytically graded and obtained from Sigma Aldrich, Germany.
AR activity was detected against DL-glyceraldehyde, by monitoring the NADPH oxidation to [NADP.sup.+] at 340 nm.
The reaction mixture consisted of 2.7 mL of potassium phosphate, 0.1 mL of NADPH, and 0.1 mL DL-glyceraldehyde. Absorbance of this reaction mixture was measured at 340 nm before and after 20 min incubation with the film strips to detect enzyme activity.
The incubation system contains lens supernatant as an AR enzyme, 0.16 mmo1/L DL-glyceraldehyde as substrate, 1.0 mmo1/L NADPH-Na4 as coenzyme, and 0.1 mol/L PBS (pH = 6.2).
The fully overlapped absorption curve in the merged figure indicated DL-glyceraldehyde did not influence [OD.sub.340nm] absorption of NADPH.
DL-glyceraldehyde can be reduced to glycerol by the AR enzyme with the conversation of coenzyme NADPH to NADP.
Human recombinant aldose reductase (HRAR) and DL-glyceraldehyde were purchased from Wako Pure Chemical Industries, Ltd.
The reaction mixture contained 0.15 mM [beta]-NADPH, 10 mM DL-glyceraldehyde, 5 [micro]l of HRAR and 100 [micro]l of test sample solution or dimethyl sulfoxide (DMSO) in a total volume of 1.0 ml of 100 mM sodium phosphate buffer (pH 6.2).
Materials: Recombinant human AR and DL-glyceraldehyde were purchased from Waco Pure Chemical Industries, Ltd.
The reaction mixtures, containing 10 mM DL-glyceraldehyde, 0.15 mM [beta]-NADPH, 5 [micro]l of the recombinant human AR and 5 [micro]l of test sample solution or DMSO in a total volume of 1 ml of 100 mM sodium phosphate buffer (pH 6.2), were incubated in advance at 25 [degrees]C for 3 min.
Materials: Human recombinant AR (HRAR) and DL-glyceraldehyde were purchased from Wako Pure Chemical Industries, Ltd.
The reaction mixture contained 0.15 mM [beta]-NADPH, 10 mM DL-glyceraldehyde, 5 [micro]l of HRAR or RLAR and 5 [micro]l of test sample solution or dimethyl sulfoxide (DMSO) in a total volume of 1 ml of 100 mM sodium phosphate buffer (pH 6.2).