5] in the southeastern United States: contrasts in sources and health associations between ascorbic acid (AA) and dithiothreitol
Twinning of sand dollar embryos by means of dithiothreitol
Purified phage suspension was dialyzed against SM buffer, mixed with loading buffer (1% SDS, 6% sucrose, 100 mM dithiothreitol
, 10 mM Tris, pH 6.
54 g dithiothreitol
, 2 g sodiumdodocyle sulfate, 8 mL of 1.
4 mM HCl, 1 mM disodium EDTA, 1 mM dithiothreitol
, and 4% protease inhibitor cocktail (Sigma) to prevent degradation.
5 mg) was reduced by dithiothreitol
, S-carbox-amidom ethylated and digested with endoproteinase C (substrate:enzyme = 50:1, w/w) in 40 [micro]l of 10 mM Tris- HCl (pH 7.
Doe & Ingalls will provide customers access to BioVectra's key bioprocessing products, such as Dithiothreitol
, TCEP, animal-free IPTG, MPEG reagents and enzymes.
When samples were treated with 400 mM dithiothreitol
(DTT) the bands of highest molecular weight observed in purified fractions of rENDOG disappeared (Figure 3A).
The extracts were prepared by diluting 5 g of honey to 10 ml with dithiothreitol
The antibody was reduced by 20 mM Dithiothreitol
(DTT) for 30 min without shaking; and then, DTT was rapidly removed by dialysis in PBS.
Diquat (dibromide monohydrate, West Chester, PA), D-mannitol, HEPES, dithiothreitol
, EDTA, phenylmethyl-sulfonyl fluoride, aprotinin, chymostatin, pepstatin A, Triton X-100, L-ornithine, pyridoxal phosphate, [alpha]-ketoglutarate, o-aminobenzaldehyde, o-phthaldialdehyde, L-arginine, L-citrulline (Sigma, Buchs, Italy) were procured from Sigma (St.
With regard to 2D-PAGE conditions, before 2D-PAGE, the strip gel was equilibrated in sample buffer (strip equilibration buffer; 6 M urea, 20% glycerol, 2% dithiothreitol
(DTT), 2% sodium dodecyl sulfate (SDS), and 375 mM Tris-HCI, pH 8.