But its activity could activated by various SH-reducing reagents, such as dithiothreitol
, GSH and [beta]- mercaptoethanol (Watanabe et al., 2003).
Briefly, the pellet was resuspended in a solution (8.3 M urea, 2 M thiourea, 64 mM dithiothreitol
(DTT), CHAPS 2% (3-((3-cholamidopropyl) dimethylammonio)-1-propanesulfonate), IGEPAL 2%, glycerol 10%, and 20 mM Tris-HCl, pH 8) and incubated overnight at 4[degrees]C in an orbital shaker.
Sperm pretreatment with dithiothreitol
increases male pronucleus formation rates after intracytoplasmic sperm injection (ICSI) in swamp buffalo oocytes.
Oxidative potential of ambient water-soluble [PM.sub.2.5] in the southeastern United States: contrasts in sources and health associations between ascorbic acid (AA) and dithiothreitol
cause the disulfide bond to break down into -SH groups .
Prior to nuclear extraction, cells (5 x [10.sup.6]/well) were washed twice with ice-cold PBS centrifuged and the pellet was suspended by adding 400 [micro]l of buffer A [10 mM HEPES (pH 7.9), 10 mM KCl, 2 mM Mg[Cl.sub.2], 0.1 mM EDTA, 1 mM dithiothreitol
and 0.5 mM phenylmethylsulfonyl fluoride (PMSF), 2 [micro]gm[L.sup.-1] of leupeptin, aprotinin, and pepstatin].
Thereafter, slides were incubated for 90 min at room temperature in 25 mM PIPES buffer containing 1 mM 1,10- phenanthroline, 1 mM dithiothreitol
, 0.014 % bacitracin, 0.1 mM captopril, 0.2 % bovine serum albumin(protease free), and 7.5 mM magnesium chloride in the presence of 200 pM [[sup.125]I]HPP-Hoe 140 (specific activity: 1212 Ci/mmol).
APPswe cells were collected, washed with ice-cold PBS and lysed with 100 [micro]l of lysis buffer (50 mM HEPES, pH 7.4, 100 mM NaCI, 0.1% CHAPS, 10% glycerol, 10 mM dithiothreitol
, 0.1 mM EDTA, 1 [micro]g/[micro]l leupeptin, 1 [micro]g/[micro]l pepstatin, 1 [micro]g/[micro]l aprotinin).
Root tissues (0.5 g) were homogenized in 50 mM KH2PO4 buffer (pH 7.5) containing 2 mM EDTA, 1.5% soluble casein, 2 mM dithiothreitol
(DTT), and 1% insoluble polyvinylpolypyrrolidone and centrifuged at 30,000 g for 20 min at 4C.
A prospective CDC-XM was performed with selected nodal T- and B-donor cells (Fluorobeads[R] T and B, One Lambda) to distinguish antiHLA Class I and II antibodies, with or without recipient serum pretreated by dithiothreitol
(DTT) to distinguish IgG and IgM antibodies.
Similarly, Vacquier and Mazia (1968a) reported that the application of dithiothreitol
to fertilized eggs of D.
Reduction studies with dithiothreitol
revealed no difference of mass between reduced and nonreduced protein.