directional cloning

directional cloning

insertion of DNA into a CLONING VECTOR in a specific orientation, by judicious use of RESTRICTION ENZYMES. Insert DNA and vector DNA are both digested with the same two different enzymes, so favouring insert to be ligated (see LIGATION into vector in a particular direction, for cloning. Alternative terminology forced cloning.

Collins Dictionary of Biology, 3rd ed. © W. G. Hale, V. A. Saunders, J. P. Margham 2005