directional cloning


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directional cloning

insertion of DNA into a CLONING VECTOR in a specific orientation, by judicious use of RESTRICTION ENZYMES. Insert DNA and vector DNA are both digested with the same two different enzymes, so favouring insert to be ligated (see LIGATION into vector in a particular direction, for cloning. Alternative terminology forced cloning.

cloning

see recombinant DNA technology.

directional cloning
the insertion of a segment of foreign DNA which has a defined polarity, e.g. different restriction enzyme sites at each end, into a plasmid vector.
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