In another sample, which was negative by culture but Gram staining showed Gram-positive
diplococci, final identification of S.
Gram stains were considered positive if they demonstrated more than 10 white blood cells per high-power field or if clue cells, Gram-negative intracellular/extracellular
diplococci, or T.
Streptococcus pneumoniae is a gram positive
diplococci with a polysaccharide capsule that helps protect it from host defense mechanisms: 90 capsular serotypes have been identified.
Instead of growing the gram-negative
diplococci typical of Neisseria, however, the cultures showed the gram-negative rods of S.
One specimen, obtained by lumbar puncture from a 1-year-old infant with acute bacterial meningitis, showed gram-positive
diplococci, and the UF-100 counted 2352 bacteria/[micro]L, but the culture remained negative after 7 days.
Urethral discharge was also seen in one patient, where gram staining revealed gram-negative intracellular
diplococci. Two men had herpes genitalis; multinucleated giant cells were found on Tzanck smear.
The presence of Gram-Negative Intracellular
Diplococci (GNID) on urethral smear is indicative of gonorrhoea infection, which is frequently accompanied by chlamydial infection.
pneumoniae colonies were identified by a-hemolysis, typical colonial morphology, presence of
diplococci on Gram stain, optochin sensitivity (Taxo P disks, Becton Dickinson) and confirmed by testing for bile solubility.
We isolated gram-negative
diplococci from both patients on modified Martin-Lewis agar (Becton Dickinson, Franklin Lakes, NJ, USA) and identified these
diplococci as N.
Gram positive
diplococci were seen on Gram stain of the pleural fluid.
Gram stain from conjunctival exudate revealed intracellular Gram negative
diplococci (see Figure 1).
In rod shape isolates, single rod-18, cluster of rod-2, while in cocci shape isolate, single cocci-10,
diplococci -5, bunch of cocci-8 and chain of cocci-24 isolates.
