Figure 3 depicts electrophoretic pattern of refolded dimeric
BMP-2 compared with that of monomeric fraction which are both eluted by 0.3 and 0.7 M NaCl, respectively.
The results indicate and confirm that the rhBMP-2 protein is present as intact dimeric
protein and is biologically active for downstream application.
However, in the presence of sodium ions, smaller populations of dimeric
forms are typically identified (Figure 6(b)).
However, the cytoplasmic sides of TMDs involve significant conformational changes, producing dimeric
interactions via TM3 and 6, resulting in interactions between the monomers in the whole regions of TM3 and TM6.
Because the PYD could mediate self-association, we were also interested in understanding the change in the dimeric
interface induced by p.
nature of the BMP2 monomer was confirmed by the native PAGE analysis and showed a distinct band at ~26 kDa [Figure 5(b)].
The octahedral [Mn[L.sup.1]([H.sub.2]O)(DCA)] entities are linked in pairs by [mu]-aquo bridges between neighbouring axial water molecules and phenoxy and methoxy oxygen atoms and also by [pi]-[pi] stacking interactions to give [[Mn[L.sup.1](DCA)([H.sub.2]O)].sub.2] dimeric
structures (Figure 8, Table 5).
This would indicate that the assay may require dissociation of antigen-antibody complexes and/or preferentially detects monomeric NS1 over its dimeric
Cl(1)#1 of 2.6300 [Angstrom]) which is achieving the dimeric
self-assembling array presented by the copper (I) complex in the solid state (Figure 3).
In the present study, we used biphalin, a dimeric
peptide [(Tyr-D-Ala-Gly-Phe-NH-)2] in which two encephalin-type pharmacophores are connected "head-to-head" by a hydrazide bridge .
It is cultivated mainly for its alkaloids, which are having anticancer effects (Jaleel et al., 2009).Catharanthus roseus, a perennial tropical tree-plant of associates Apocynaceae, produces a high number of monoterpenoidindole alkaloids of which two dimeric
alkaloids, vincristine, and vinblastine are clinically useful oncolytic drugs (Cordell 1980).
It is a dimeric
glycoprotein and a 140-kilodalton (kDa) hormone.