digitonin


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dig·i·to·nin

(dij'i-tō'nin),
1. A steroid glycoside obtained from Digitalis purpurea that has no cardiac action; used as a reagent in the determination of plasma cholesterol and steroids having a 3-hydroxyl group in beta configuration.
2. A mixture of four different steroids found in the seeds of Digitalis purpurea; a strong hemolytic poison. They can act as nonionic detergents in the solubilization of membrane proteins.
Synonym(s): digitin
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References in periodicals archive ?
The molecular weights of the respiratory complexes or supercomplexes were estimated by using digitonin bovine heart mitochondrial complexes as standard: single complex: I = 1,000 kDa, V = 750 kDa, III2 = 500 kDa, IV = 230 kDa, II = 130 kDa; supercomplexes: I-III-[IV.sub.1-4] = 1500-2100 kDa, [V.sub.2] = 1500 kDa.
The cells were then pelleted by centrifugation for 3min at 1700g and 4[degrees]C; gently suspended in permeabilization buffer (220 mM mannitol, 68 mM sucrose, 80 mM KCl, 10 mM HEPES (pH7.4), 2.5 mM EGTA, 1 mM EDTA, 1 mM DTT, 200 (g/ml digitonin, and 1% protease inhibitor cocktail); placed on ice for 5 min; and centrifuged for 5min at 750g and 4[degrees]C.
Osteosarcoma cybrids were permeabilized with digitonin (4 [micro]M), and respiration was initiated with complex I substrate (malate/pyruvate, 5 mM).
Digitonin (80 [micro]M) was used as a positive control, and untreated cells were used as negative controls.
The positive control was cells that were incubated with 50 [micro]g/mL digitonin (Amend Drug and Chemicals Co., USA) for 5 min, as previously described (30).
After treatment, the cell pellet after centrifugation was lysed with Tris buffer (50 mol/L Tris-HCl, 1 mmol/L EDTA, and 10 mmol/L EGTA at pH = 7.4) containing 10 [micro]mol/L digitonin for 20 min at 37[degrees]C.
Synergism of three-drug combinations of sanguinarine and other plant secondary metabolites with digitonin and doxorubicin in multi-drug resistant cancer cells.
Frischknecht, "Synergistic and additive effects of epigallocatechin gallate and digitonin on Plasmodium sporozoite survival and motility," PLoS ONE, vol.
Behavior of polymer supported digitonin with cholesterol in the absence and presence of butter oil.
However, its cells are sensitive to digitonin. The presence of only plasma membrane makes it vulnerable to osmotic shock and the effect of detergents [21, 29].
Digitonin sensitivity test was carried out to differentiate between Mycoplasma and Acholeplasma genera using filter paper discs impregnated with 0.2 mL of 1.5% (W/V) ethanol solution of digitonin and dried overnight.