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However, the direction of change was the same in the estrous and diestrous phases, that is, hormonal level had an insignificant effect on emotional behavior, locomotor, and exploratory activity.
Diestrous phase occurrence significantly increased in treated rats (19.40 [+ or -] 0.65 days) compared to control (13.10 [+ or -] 0.38 days).
This was evident by the significant increase in the average number of days for the diestrous compared to the control and a significant reduction in the average number of days for estrous compared to the control.
The estrous cycle stage was evaluated before and after treatments and determined in pro-estro (Pro.), estrus (Est.), diestrous (Diest.), and metaestrous (Met.).
In experiment 1, males and diestrous females received vehicle or 1, 3, 9, or 27 mg TBBPA (n = 7 per dose).
At the onset of darkness on the fourth day, males and diestrous females received a sc injection of vehicle or 1 mg TBBPA (corresponding to 33.8 [+ or -] 3.7 mg TBBPA/kg for females and 23.4 [+ or -] 2.4 mg TBBPA/kg for males) dissolved in 0.05 mL peanut oil (n =15 per dose).
In addition, intact estrous females showed more antinociception than diestrous females .
Cytochemical detection of GnRH binding sites on rat pituitary cells with LH, FSH, and GH antigens during diestrous upregulation.
It was observed the phase of diestrous with 15% in group II, and 23,5% in group III, not being observed the phases of proestrous and metaestrous.
Also, it was observed in these groups the diestrous phase with percentage of 15,00% in group I, and 23,50% in group III, not being observed the proestrous and metaestrous phases (Fig.
The predominance of mucus and leucocytes in the smear was considered indicative of metaestrous; the presence of high concentrations of leucocytes was indicative of diestrous; proestrous was indicated by the observation of many nucleated epithelial cells, and the presence of many keratinized cells indicated the estrous phase.
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