Cellular and mitochondrial free radical concentration: The diesterase
bond of DCFDA is cleaved in living cells to produce dichlorofluorescin (DCF) which is oxidized by cellular free radicals (14,21,24).
AI172064 56646 Lgals1 Lectin, galactose 3' binding, soluble 1 J02810 24423 Gstm1 Glutathione S-transferase, mu 1 X04229 H32189 565355 50672 Ednrb Endothelin receptor type B X57764 AA818970 U09540 25426 Cyplbl Cytochrome P450 1b1 X83867 AI176856 M14972 50549 Cyp4a10 Cytochrome P450, 4a10 AA924267 D83538 64161 Pik4ca Phosphatidylinositol 4-kinase U39572 J04563 24626 Pde4b Phosphodiesterase 48, cAMP-specific [dunce (Drosophila)- homolog phospho- diesterase
E4] M25350 X81395 29225 Cesl Carboxylesterase 1 U10697 (a) http:/www.
showed that PTX can also increase sperm motility by inhibiting the activity of phos-phodiasterase diesterase
enzyme to increase the intracellular concentration of cAMP, glycolysis and production of energy (34).
Nucleotide pyrophosphatases/Phospho- diesterases
(NPPs, ecto-NPPs or PDs-I) are composed of a group of multi-gene family of metalloenzymes, which catalyze the release of nucleoside-5'-monophosphates from hydrolysis of a variety of nucleotide derivatives .
Quercetin reduces basophil degranulation, (109), (110) and histamine release, (111-113) and dampens the inflammatory response directly by modulating certain key enzymes including lipoxygenase, phospholipases, and nucleotide diesterases