(4) Since the introduction of dideoxynucleoside
reverse transcriptase inhibitors (NRTIs) in 1985 and after the approval of the first protease inhibitor in 1995, the availability of new fixed-dose combinations regimens of new antiretroviral drugs, evolved faster in the developed world.
The PC12 cell as a model for studies of the mechanism of inducing of peripheral neuropathy by anti-HIV-l dideoxynucleoside
In vitro models to predict the in vivo mechanism, rate and extet of placental transfer of dideoxynucleoside
drugs against human immunodeficiency virus.
Lamivudine (3TC), the negative enantiomer of 2'-deoxy-3'thiacytidine, is a dideoxynucleoside
analogue used in combination with other agents in the treatment of human immunodeficiency virus type 1 (HIV-1) infection and as monotherapy in the treatment of hepatitis B virus (HBV) infection.
Assay-specific deoxynucleoside triphosphates (dATP, dGTP, dTTP; Amersham) and dideoxynucleoside
triphosphates (ddA, ddC, ddG, ddT; BioLog Life Science Institute) were added at 0.12 mmol/L.
Therefore, in that study, a very short extension strategy using alternating deoxynucleoside triphosphates (dNTPs) and dideoxynucleoside triphosphates was adopted so that the products differed in mass by [M.sub.r] 300 to 400 to resolve the 50/50 heterozygote for direct quantification from mass spectra.
Primer extension reactions were carried out in a 20-micro]L volume containing 50 ng of input PCR product and 0.75 [micro]M primer, 25 [micro]M each of the dideoxynucleoside triphosphates (Amersham Pharmacia Biotech), and 0.5 U of ThermoSequenase DNA polymerase (Amersham Biosciences) in 1 x supplied reaction buffer.
Our strategy for detecting the splice variants expressed from the genes is based on the extension of gene-specific minisequencing primers with fluorescent dideoxynucleoside
triphosphates (ddNTPs), each of which define a specific splice variant (see Table 52 in the online Data Supplement).
DNA polymerase extends it by adding one fluorescently labeled dideoxynucleoside
triphosphate complementary to the variant base.
Comparison of mitochondrial morphology, mitochondrial DNA content, and cell viability in cultured cells treated with three anti-human immunodeficiency virus dideoxynucleosides
. Antimicrobial Agents and Chemotherapy, 38, 1824-1828.
The primer extension reaction was performed using modified thermostable DNA polymerase in the presence of dideoxynucleosides
; the reaction products were analyzed using MALDI-MS.