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Bisected embryos were placed on modified MS (Murashige and Skoog, 1962) inorganic salts, with 1.25 mg [L.sup.-1] Cu[SO.sub.4], 1 g [L.sup.-1] casein hydrolysate, 0.25 g [L.sup.-1] myo-inositol, 30 g [L.sup.-1] maltose, 4.5 g [L.sup.-1] 2,4 dichlorophenoxyacetic acid
(2,4-D), and solidified with 3.5 g [L.sup.-1] Phytagel (Sigma, St.
Early commercial products containing 2,4,5-T (trichlorophenoxyacetic acid) alone or in combination with 2,4-D (dichlorophenoxyacetic acid
) were found to have dioxin and dioxin-like contaminants.