Literature shows the use of different solvents for the PCDDs extraction from various matrices prior to quantitative GC analysis, for example, n-hexane:acetone (80:20, v/v) for fruits and vegetables [13],
dichloromethane:n-hexane (1:1, v/v) for fish, beef and corn silage [5], diethyl ether:n-hexane (7:10, v/v) followed by n-hexane for milk [38] and toluene for chicken eggs [29].
In case of the
dichloromethane extract (CAD), the sample showed 22.32%, 21.99%, and 14.87% inhibition against E.
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dichloromethane extract (18.53 [+ or -] 2.42 mM TEAC).
Additionally, the hydrocarbon (
dichloromethane soluble part) revealed that the sample contains paraffinic-based hydrocarbons (25.0 wt%) suggesting that it is the mixture of diesel and lube oil.
The extraction of transition metal ions with [H.sub.8]L from the aqueous phase, containing 0.1 M ethylenediamine [[[C.sub.2][H.sub.4](N[H.sub.2]).sub.2]] and 0.1 M trimethylenediamine [[(C[H.sub.2]).sub.3][(N[H.sub.2]).sub.2]] instead of ammonia, into the
dichloromethane solution was also examined.
In another attempt, morpholine produced similar signal abundance in two different samples (20mg x [L.sup.-1] in
dichloromethane without derivatization and 400 [micro]g x [L.sup.-1] in pure water after derivatization) (Figures 5(b) and 5(c)).
The plant in general is a strong antigonorrhea agent with n-hexane extract being the most compared to other extracts (
dichloromethane, methanol, and water extracts).
Dichloromethane was found in large amounts, and so was 1,2-dichloroethane, an ozone-depleting substance used to make PVC.
The concentrations of the two targeted compounds were obtained by using the liquid-liquid extraction method: 100 mL of the sample was transferred into a conical flask and mixed with 100 mL of
dichloromethane. The mixtures were shaken in a shaking incubator at room temperature for two hours with 120 rpm of shaking speed.
Dichloromethane was used as extraction solvent (15 mL).
In our previous experiments we found that MP aqueous extract could lower the toxicity of GE
dichloromethane extract [13].