biphasic positive test indicative of hepatic jaundice on application of Ehrlich diazo reaction
Total bilirubin level was determined by diazo reaction [16, 17].
Nosslin, "The direct diazo reaction of bile pigments in serum," Scandinavian Journal of Clinical and Laboratory Investigation, supplement 49, pp.
The diazo reaction
of bilirubin and bilirubin diglucuronide.
(3) published a method for measuring total serum bilirubin and its fractions by use of bilirubin oxidase (BO) and the diazo reaction
. Among those fractions was the sum of mono- and diglucuronide (CB).
(3) identified novel oxidative metabolites of bilirubin in human urine as substances that are recognized by an anti-bilirubin monoclonal antibody, 24G7, but are negative in the diazo reaction
. These metabolites are tripyr-role biocompounds and are designated biopyrrins.
To visualize AST activity, the membrane was stained with an AST isoenzyme reagent staining kit (Wako Pure Chemical Co.) consisting of 60 mmol/L Tris-HCI, pH 7.4 (1.4 mL), 0.1 mol/L sodium L-asparaginase (0.8 mL), 0.1 mol/L [alpha]-ketoglutaric acid (0.2 mL), 1 g/L pyridoxal-5 phosphate (0.05 mL), and Fast Violet B salt, 3 mg; a principle of this procedure is the diazo reaction
. The patient's serum produced a single band on the membrane between supernatant AST (s-AST) on the anodal side and mitochondrial AST (m-AST) on the cathodal side, suggesting that something was bound to AST.
To test the possibility of a diazo reaction
with etoposide, we added increasing amounts of etoposide to a control urine and measured the apparent VMA.