It is likely that the N atom in 4-fused ring is important for the diaphorase
In vivo effect of Daonil on erythrocyte NADH diaphorase
activity of rat at pH 8.0 and 30[degrees]C NADH (iu/L) Daonil (mg/200g Day 1 Day 2 body weight) X [+ or -] SD X [+ or -] SD 0.00 6.13 (a) 6.19 [+ or -] 1.24 [+ or -] 0.81 0.01 6.66 (a) 7.83 (b) [+ or -] 0.83 [+ or -] 0.59 0.02 7.00 (b) 8.50 (c) [+ or -] 0.02 [+ or -] 0.01 0.03 7.43 (b) 9.41 (d) [+ or -] 0.01 [+ or -] 0.14 Daonil (mg/200g Day 6 Day 14 body weight) X [+ or -] SD X [+ or -] SD 0.00 6.70 (a) 6.91 (b) [+ or -] 0.65 [+ or -] 0.42 0.01 10.17 (e) 10.35 (e) [+ or -] 0.69 [+ or -] 0.96 0.02 10.35 (e) 11.01 (f) [+ or -] 0.97 [+ or -] 1.41 0.03 11.44 (f) 7.04 (b) [+ or -] 0.82 [+ or -] 1.03 Table 2.
however we hypothesized that since our patient might be suffering from diaphorase
deficiency, even doses that are described as safe for other patients might be toxic due to the inefficient reducing system.
The samples were then mixed with 100 [micro]g/ml ADH, 10 [micro]mol/L FMN, 20 [micro]mol/L resazurin, 10 mmol/L nicotinamide, 10 [micro]g/ml diaphorase
, 2% ethanol, 0.1 mg/ml BSA, and 100 mmol/L sodium phosphate, pH 8.0, and incubated in the dark for 4 h at room temperature.
Hereditary methemoglobinemic cyanosis due to diaphorase
deficiency in three successive generation.
After the addition of 300 [micro]l of a reaction cocktail, consisting of 50 mM TEA (pH 8.1), 0.02% BSA, 50 mM KC1, 0.5 mM [MgCl.sub.2], 670 [micro]M ATP, 0.12 [micro]M [NADP.sup.+], 25 [micro]M resazurin sodium salt, 5.5 units/ml hexokinase, 16 units/ml G6PDH, and 1 unit/ml diaphorase
, the mixture was incubated for 90 min.
Talalay, "Persuasive evidence that quinone reductase type 1 (DT diaphorase
) protects cells against the toxicity of electrophiles and reactive forms of oxygen," Free Radical Biology and Medicine, vol.
2DG, hexokinase, glucose-6-phosphate dehydrogenase, diaphorase
, rezazurine, ATP, [NADP.sup.+]+, and all the primers were obtained from Sigma Aldrich, Bangalore, India.
assay and NOS immunohistochemistry
This differentiation is based on the concentration of oxireductases, especially diaphorase
oxireductases such as nicotinamide adenine dinucleotide tetrazolium reductase (NADH-TR), in muscle tissues (Dubowitz & Brooke, 1973).
18.104.22.168); gels with pH gradients 3 to 7 (50%) and 4 to 5 (50%) were used for diaphorase
Pharmacological and physiological studies have provided evidence that nitric oxide (NO) is the primary mediator in nonadrenergic, noncholinergic relaxation of the gastrointestinal tract.[15,16] Enzymes responsible for NO synthesis constitute a family with at least 3 distinct isoforms: inducible, endothelial, and neuronal NO synthase (NOS). Neuronal NOS (nNOS) is the isoform that is expressed in the myenteric plexus of the gastrointestinal tract.[18,19] Scherer-Singler et al described the enzymatic reduction of nitroblue tetrazolium to an intensely blue, water-insoluble salt by nicotinamide adenine dinucleotide phosphate (reduced form, NADPH) diaphorase
in a population of neurons.