Blood urea was done manually by DAM METHOD(diacetylmonoxime
) reference range 14-40mg/dl, (11) Serum Creatinine reference range 0.61.mg/dl, Urinary Creatinine reference range 1-2gm/day was done manually by JAFFE'S METHOD (12) using Colorimeter, and Urinary protein measurement was done using a kit method which is basically colorimetric method.
Urea was measured by the direct diacetylmonoxime method modified by Mulvenna and Savidge (1992).
A modified manual method for the determination of urea in seawater using diacetylmonoxime reagent.
An improvement in determination of urea usng diacetylmonoxime
method with and without deproteinization.
Recently new series of Co(II), Ni(II), Cu(II), Cd(II), and Hg(II) complexes were synthesized by the condensation of naphthofuran-2carbohydrazide and diacetylmonoxime. The ligand along with its metal complexes has been characterized on the basis of analytical data, IR, electronic, mass, *HNMR, ESR spectral data, thermal studies, magnetic susceptibility, and molar conductance measurements.
Halli, "Metal (II) complexes derived from naphthofuran-2-carbohydrazide and diacetylmonoxime Schiff base: synthesis, spectroscopic, electrochemical, and biological investigation," Bioinorganic Chemistry and Applications, vol.
Prompted by the biological importance and applications of the above compounds in various fields, the present work is devoted to the synthesis and characterization of Schiff base ligand obtained by the condensation of naphthofuran-2-carbohydrazide with diacetylmonoxime
(Scheme 1) and its metal complexes by using metal ions like Co(II), Ni(II), Cu(II), Cd(II), and Hg(II).
A colorimetric assay based on the reactivity of carbamoyl groups with diacetylmonoxime
may be used (22).
Estimation of serum Urea: Urea reacts with hot acidic Diacetylmonoxime
in the presence of Thiosemicarbazide and produces a rose-purple coloured complex, which is determined colorimetrically .
Total protein was determined by Biuret method using Total Protein Kit from Crest Biosystems (Doumas, 1975), Albumin by BCG method using Albumin Kit from Crest Biosystems (Doumas, 1971) and Urea by Diacetylmonoxime
(DAM) method using Urea Kit from Span Diagnostics ltd (Wybenga, 1971).
In this procedure, diacetylmonoxime
, thiosemicarbazide, and the acid reagent were mixed on-line and combined with the sample carrier prior to the heating bath (95[degrees]C) and detector.
Urea concentration in urine or serum was measured using the diacetylmonoxime
method of Marsh et al.