Dextransucrase from Leuconostoc-paramesenteroides was immobilized using different immobilization techniques.
Keywords: Dextransucrase, enzyme immobilization, calcium alginate, Leuconostoc.
Immobilization of dextransucrase via entrapment in alginate provided high and good operational stability.
Immobilization of dextransucrase in alginate fibers resulted in 90% relative activity .
Immobilization of dextransucrase from Leuconostoc mesenteroides PCSIR-4 on alginate is optimized for application in the biosynthesis of dextran from sucrose.
In this work, different immobilized dextransucrase enzyme preparations were made and investigated the relative performances such as the reusability.
Dextransucrase activity was determined by measuring the initial rate of fructose production using the dinitrosalicylic acid method (DNS method).
The reaction mixture without dextransucrase was incubated for 5 min in a thermostated stirred tank reactor at 30degC.
In this experiment the effect of temperature of the reaction on dextransucrase activity was studied using different temperatures ranging from 20 to 80degC.
A rapid screening procedure; strains produce the high proportion of alternansucrase to dextransucrase
and of alternan to dextran either fermentatively by cultivation on sucrose, or enzymatically by using the alternansucrase that results from this invention.