A porphyrin derivative resembling the protoporphyrins except that the two vinyl side chains are replaced by hydrogen.
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The calibrators included coproporphyrin III dihydrochloride, uroporphyrin III dihydrochloride, 2-vinyl-4-hydroxymethyl deuteroporphyrin IX dihydrochloride, deuteroporphyrin IX, protoporphyria IX, and a porphyrin marker kit containing a mixture of uroporphyrin, coproporphyrin, and hepta-, hexa-, and pentacarboxylic acid porphyrins of the I isomer series.
The internal standard (2-vinyl-4-hydroxymethyl deuteroporphyrin IX) was dissolved in 6 mmol/L HCl and diluted with deionized water to give a final concentration of 5 [micro]mol/L.
Mixed calibrators of free porphyrins (uroporphyrin I, heptacarboxylic porphyrin I, hexacarboxylic porphyrin I, pentacarboxylic porphyrin I, coproporphyrin I, and mesoporphyrin IX), deuteroporphyrin, i-urobilin, and stercobilin were purchased from Porphyrin Products.