A porphyrin derivative resembling the protoporphyrins except that the two vinyl side chains are replaced by hydrogen.
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To this end, we investigated whether the microinjection of zinc deuteroporphyrin 2,4-bis glycol (ZnDPBG, an HO inhibitor), heme-lysinate (substrate overload) or the selective inhibitor of soluble guanylate cyclase (1H-(1,2,4) oxadiazolo [4,3-a] quinoxaline-1-one (ODQ)) into the LC in different groups of rats produces alterations in emotional behavior as assessed by the elevated plus maze test (EPM) and light-dark box test (LDB) in rats.
The calibrators included coproporphyrin III dihydrochloride, uroporphyrin III dihydrochloride, 2-vinyl-4-hydroxymethyl deuteroporphyrin IX dihydrochloride, deuteroporphyrin IX, protoporphyria IX, and a porphyrin marker kit containing a mixture of uroporphyrin, coproporphyrin, and hepta-, hexa-, and pentacarboxylic acid porphyrins of the I isomer series.
The nonselective HO inhibitor zinc deuteroporphyrin 2,4-bis glycol (ZnDPBG, 50 nmol/4 [micro]L) and hemin used in this study were obtained from Porphyrin Products (Logan, USA).
Mixed calibrators of free porphyrins (uroporphyrin I, heptacarboxylic porphyrin I, hexacarboxylic porphyrin I, pentacarboxylic porphyrin I, coproporphyrin I, and mesoporphyrin IX), deuteroporphyrin, i-urobilin, and stercobilin were purchased from Porphyrin Products.