A compound derived from biotin by the removal of the sulfur atom; a precursor of biotin in bacteria and molds; it can substitute for biotin in some microorganisms, but is without effect on or is inhibitory to the growth of others.
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5 mmol/L desthiobiotin (Sigma) at a flow rate of 0.
The protein could be purified in a single step by affinity binding to a StrepTactin column; on washing with desthiobiotin, the protein eluted as a single band with an estimated molecular mass of 87 kDa (Fig.