The gels can be stained using Comassieblue and then partially destained
to promote better contrast to the heavily stained insulin species (Figure 9A).
0 mm mature female, collected with holotype (cleared and stained with alcian and alizarin; only cranium, pectoral girdles and axial skeleton, completely destained
by September 2012.
Specimens were stained in aceto-carmine, destained
in 70% acidulated alcohol until light pink, and then dehydrated in a stepwise manner with 1 h each in the following concentrations of ethanol: 85%, 95%, 100%, and 100% again.
Gels were stained with 1% Coomassie blue R250 for 1 hr and was destained
(45% methanol, 10% acetic acid).
5-2 mm, washed, destained
, and digested with 20 g/L trypsin.
5% (w/v) amido black, and destained
in 50% methanol containing 5% (v/v) acetic acid.
After staining, the gel was destained
in 5% acetic acid.
1 % w/v Coomassie Brilliant Blue R-250 (Sigma) in methanol:water:ethanoic acid (5:5:1) and then destained
with a solution of methanol:water:ethanoic acid (1:17:2).
Although it requires overnight staining for best results, the method is straightforward and the gels do not need to be destained
The gel was further stained with a 40% (v/v) staining solution for 24 hours and destained
with a 40% (v/v) destaining solution for 3 hours.
with Syber Green (Invitrogen, USA) 1:10,000 and destained
in acetic acid for 15 min.
Gels were run for 6 h and were stained with ethidium bromide for 20 min and destained
in distilled water for 30 min.