In-gel sample is separated from SDSPAGE gel and destained
before tryptic digestion.
(D) Colonies stained with crystal violet were destained
, and absorbance was measured on a plate reader at 584 nm; values represent the ratio of absorbance to the number of CFUs.
The gels can be stained using Comassieblue and then partially destained
to promote better contrast to the heavily stained insulin species (Figure 9A).
Further, the gel was stained with Coomassie brilliant blue R250 for 2 h and destained
overnight to reveal the bands.
In-Gel Trypsin Digestion and MALDI-TOF-TOF-MS Analysis Gel band 1-1 was destained
, dehydrated, and dried under vacuum.
The following day, to visualize the bands, gels were stained with Coomassie Brilliant Blue Dye (Bio-Rad) for 3hrs and destained
with Destaining Solution for 45 min.
After staining the gels were destained
until the clearance of blue background.
The gel was heated in the Amaranth staining solution at 70degC for 10 minutes or left overnight in the staining solution at room temperature, and was washed with the destaining solution by a gap of 15 minutes to produce clear red bands on the colorless gel while the other portion of the resolved gel was stained overnight with Coomassie brilliant blue R250 and destained
by the normal procedure followed by lameli ; washed several times with the destaining solution for 24 hour.
The cleared and stained material of this specimen, prepared while it was still relatively fresh, retained its stain for at least one year, but by 2012 it was completely destained
. In 2012 a paratype of L.
Specific protein bands were excised from the gel matrix, destained
with SilverSNAP destaining kit (Pierce, Rockford, IL), and subjected to in-gel tryptic digestion as previously described  (Figure 1(d)).
After staining the gel was destained
by washing twice in destaining solution (20% acetonitrile, 50 mM sodium acetate, and pH 4) for 30 minutes each time.
The plates were destained
for approximately 10 min with an aqueous solution of 10% acetic acid and 2% dimethylsulfoxide.21