References in periodicals archive ?
Destain, "A real-time grading method of apples based on features extracted from defects," Journal of Food Engineering, vol.
Dauphin DR, Destain J, Fauconnier ML, Lognay G, and Thonart P, International Journal of Microbiology, 1-9 (2010).
Protein spots were excised from stained 2D gels, destained, digested with trypsin (sequence grade, Pro-mega, Madison, USA) at 37 [degrees]C overnight (6ng/[mu]l).
The gel was then dried for 5 min, stained in acid violet for 5 min, destained, and finally dried.
After the gels were dried at 65[degrees]C for 10 min, they were stained with 5 g/L amido schwarz in a 50 mL/L acetic acid solution for 4 min and destained before a final drying at 75[degrees]C for 8 min.
The sample lanes were destained in methanol, rinsed in Tris-buffered saline with Tween 20 (TBST) buffer (containing 10 mmol/L Tris-HCl pH 7.5, 150 mmol/L NaCl, and 0.5 mL/L Tween 20), and incubated for 30 min with 1:10 diluted cow colostrum in TBST buffer.
Gels were destained in 50 mL/L acetic acid for at least 2 h.
Nations who do not rise are destained to dust bin of history.
After incubation, gel was stained with coomassie brilliant blue R250 for 30 min and then destained in 5% methanol, 5% glacial acetic acid.
The gels were destained with 40% v/v ethanol and 10% v/v acetic acid until visualization of spots.
Paraffin slices were dewaxed and rehydrated, then stained with hematoxylin (Beyotime Biotechnology, China) for 3 min, washed for 5 min, destained with hydrochloric acid alcohol, washed for 5 min, and fast stained with eosin (Beyotime Biotechnology, China).
The resolved protein was either stained using Coomassie blue reagent overnight and destained with 50% dd[H.sub.2]O, 40% methanol and 10% acetic acid before imaging or transferred to a nitrocellulose membrane using the TransBlot-Semidry Transfer System (Bio-rad) for the Western blotting.
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