Antibodies to uroplakin III (UROIII), S100P, and GATA-3 have been shown to be urothelial-associated markers, while expression of CK14 and desmoglein-3 has been associated with squamous differentiation.
The napsin A, S100P, surfactant protein A, UROIII, thrombomodulin, CK14, desmoglein-3, and S100A1 immunostaining procedures were performed at Cedars-Sinai Medical Center with either a BenchMark ULTRA, Leica BONDMAX (Leica Microsystems, Wetzlar, Germany), or Dako Auto-stainer (Dako North America, Inc, Carpinteria, California) automated staining platforms.
In contrast, CK14 (77% versus 32%) and desmoglein-3 (87% versus 11%) were more likely to be positive in cases of primary pulmonary invasive SCC than invasive UCa of the bladder.
The paired bladder lesion from case 8 was negative for CK7, CK20, GATA3, CK14, desmoglein-3, and UROIII.
Results from the initial evaluation of invasive bladder UCa and primary pulmonary SCC demonstrated clear staining differences between the 2 tumor types with immunostains for CK7, CK20, GATA-3, CK14, desmoglein-3, and UROIII.