It was applied by preparation of the toxins and their treatment , application of dermonecrotic
test I/D of an albino guinea pig [12, 13] and interpretation of the results according to colour degree of the dermonecrotic
reaction and its neutralization .
The diagnosis of brown recluse spider bite is overused for dermonecrotic
wounds of uncertain etiology.
Adult guinea pigs weighing 350-450 g (used for the dermonecrotic
reaction) in addition to immature BALB/c mice with an average weight of 15-20 g (used for the toxin neutralization test), were maintained in air-conditioned (22 [+ or -] 1[degrees]C) quarters under uniform husbandry conditions with a light cycle from 06:00 to 20:00.
lesions, Loxosceles venom causes an immediate vascular constriction at the bite site.
Available data suggest both a hydrophilic, neurotoxic component and a hydrophobic, dermonecrotic
component (Samet et al.
Oligosaccharide residues of Loxosceles intermedia (brown spider) venom proteins: dependence on glycosylation for dermonecrotic
CNF1, CNF2, and the dermonecrotic
toxins from Bordetella species form a unique subset in this family, since these toxins have the capacity to activate Rho (Figure 1 C) (29-32).
Catalase_test ; Gelatin liquefaction test; indole test;nitrate reduction test ;Sugar_fermentation_test; hydrogen Sulphide production test (Oxoid) and litmus milk_medium : Nagler's test by half antitoxin plate : Typing of Clostridium perfringens toxins dermonecrotic
test in albino guinea pigs Toxin antitoxin neutralization test : Determination of the MLD of the prepared toxin : Antibiogram sensitivity test (Oxiod): Pathological, gross and histopathological examination
These factors include toxins such as adenylate cyclase toxin, dermonecrotic
toxin, the type III secretion system, and adhesins such as filamentous hemagglutinin, pertactin, and fimbriae [15, 16].
These effects range from carcinogenic and nephrotoxic health effects to dermonecrotic
and immunosuppressive health effects (Orriss 1997).
In mice, only lethal, dermonecrotic
and myotoxic activity was detected with P.
Although acute dermonecrotic
lesions of fish have been induced in laboratory studies of P.