Folding of 8-17 deoxyribozyme
studied by three-color alternating-laser excitation of single molecules.
Factors that influence deoxyribozyme
cleavage during polymerase chain reaction.
The deoxyribozymes will provide a means of synthesizing (ligating) large amounts of RNA in hours at 37[degrees]C.
The deoxyribozymes require the RNA junction ligation termini to be a 2'-3' cyclic phosphate and a 5' hydroxyl group.
Functionally, these deoxyribozymes
are cousins of protein enzymes known as restriction endonucleases.
Although the activity of the described oligonucleotides is weak, these deoxyribozymes
could be further optimized to enhance their catalytic power, making them valuable for diagnostic applications.