Considering phytic acid antioxidant activity, its various applications and its high concentration in rice bran, this study had the objective of evaluating the antioxidant capacity of purified phytic acid from rice bran using the FRAP, BPS, and deoxyribose
E., 1987, "The deoxyribose
method: a simple "test tube" assay for determination of rate constants for reactions of hydroxyl radicals", Analytical Biochemistry., 165, pp.
Result shows the abilities of the extract and standard thiourea to inhibit hydroxyl radical-mediated deoxyribose
degradation in a Fe[Cl.sub.3]-EDTA-ascorbic acid and [H.sub.2][O.sub.2] reaction mixture.
ROS induces damage to cellular DNA, including the rupture of deoxyribose
rings, the appearance of apurinic and apyrimidinic sites, single-and double-strand breaks, DNA protein crosslinks, and formation of oxidized bases .
Hydroxyl radical ([sup.*]OH) was determined by the inhibition of deoxyribose
Then, the deoxyribose
hydroxyl group at the broken end attacks the enzyme-DNA linkage, forming the DNA phosphodiester linkage and freeing the enzyme for the next round of reactions (Wang 1994).
Each nucleotide (A, G, C, and T) contains 3 components [ILLUSTRATION FOR FIGURE 1 OMITTED]: a deoxyribose
(a five-carbon sugar), a triphosphate group, and a base, which can be adenine, thymine, guanine, or cytosine.
It is well known that neither [O.sub.2.sup.*-] nor [H.sub.2][O.sub.2] is capable of interacting with either deoxyribose
or the base portions of DNA; this suggests that the secondarily derived *OH radical may be the primary reactive species.
They are composed of sugars (deoxyribose
), phosphate groups, and nucleobase attached to the sugars.
assay, erythrocyte membrane peroxidation and rat liver microsomal lipid peroxidation induced by [Fe.sup.2+]/ascorbate (Hosseinzadeh et al.
A Structure for Deoxyribose
Nucleic Acid," published in Nature in 1953, the two scientists described the double-helical form of the molecule, shaped like a twisted ladder, in which each rung is made up of four nucleotides: adenine, thymine, cytosine, and guanine (typically abbreviated as A, T, C, and G).
Its labile phosphodiester backbone and the tendency for base cleavage from deoxyribose
makes intact volatilization of larger strands challenging [181.