The focus of this research was to study 2-hydroxy-1,4-dioxane, a by-product of [alpha]-hydroxy-N-nitrosomorpholine, and to determine its effect on deoxyguanosine
Clinical and molecular features of mitochondrial DNA depletion due to mutations in deoxyguanosine
For example, the first G peak is twice as tall as the first T peak, indicating that 2 consecutive deoxyguanosine
triphosphates (underlined) were incorporated, whereas only one deoxythymidine triphosphate (underlined) was incorporated; 1X and 2X lines represent peak heights because of single or double base incorporation events, respectively.
the formation of 8-hydroxy-2'- deoxyguanosine
(8-OHdG), the most studied form of base modification, can be quantified using specific antibody by enzyme-linked immunosorbent assay.
8-OHdG is a by-product from damaged DNA when it is attacked by hydroxyl free radicals at deoxyguanosine
in DNA (Park and Floyd 1992).
) is an oxidized derivative of deoxyguanosine
Lymphocyte-selective cytostatic and immunosuppressive effects of mycophenolic acid in vitro: role of deoxyguanosine
5mM magnesium chloride, 200 [micro]M each of deoxynucleoside triphosphates (deoxyadenosine triphosphate, deoxycytidine triphosphate, deoxyguanosine
triphosphate, and deoxythymidine triphosphate), 0.
Under healthy physiological conditions, the role of dCK is the phosphorylation of deoxycytidine, deoxyadenine, and deoxyguanosine
to their monophosphate forms.
5 mmol/L magnesium chloride (Perkin-Elmer); 200 pmol/L each of deoxyadenosine triphosphate, deoxycytidine triphosphate, deoxyguanosine
triphosphate, and deoxythymidine triphosphate (Sigma); 200 pmol of each primer; and 10 [micro]L of the extracted DNA sample.
Moreover, oxidative markers, leukocyte 8-hydroxy-2'- deoxyguanosine
and lipid hydroperoxides, are detected in association with long-term and short-term clinical outcomes, respectively, after spontaneous ICH [15, 16].
Furthermore, a previous study suggested that artifactual oxidation of deoxyguanosine
can occur during sample preparation (33).