Clinical and molecular features of mitochondrial DNA depletion due to mutations in deoxyguanosine
After the administration of green tea catechin, we observed decreased rates of staining for 4-HNE, an indicator of hepatic lipoperoxidation, and 8-OHdG, an indicator of oxidative injuries of deoxyguanosine
or a DNA constituent.
5mM magnesium chloride, 200 [micro]M each of deoxynucleoside triphosphates (deoxyadenosine triphosphate, deoxycytidine triphosphate, deoxyguanosine
triphosphate, and deoxythymidine triphosphate), 0.
Lymphocyte-selective cytostatic and immunosuppressive effects of mycophenolic acid in vitro: role of deoxyguanosine
5 mmol/L magnesium chloride (Perkin-Elmer); 200 pmol/L each of deoxyadenosine triphosphate, deoxycytidine triphosphate, deoxyguanosine
triphosphate, and deoxythymidine triphosphate (Sigma); 200 pmol of each primer; and 10 [micro]L of the extracted DNA sample.
Under healthy physiological conditions, the role of dCK is the phosphorylation of deoxycytidine, deoxyadenine, and deoxyguanosine
to their monophosphate forms.
Selective inhibition of PNP causes select nucleosides, including deoxyguanosine
, to accumulate and be converted to deoxyguanosine
1% Triton X-100 (Nacalai Tesque, Kyoto, Japan); 2% formamide; 10 pmol of primer pairs; 200 [micro]mol each of deoxyadenosine triphosphate, deoxyguanosine
triphosphate, deoxycytidine triphosphate, and deoxythymidine triphosphate; and 2.
The amount of OH8dG, expressed as a ratio of the amount of deoxyguanosine
(dG) or as fmol/micrograms of DNA, increased progressively with normal aging in both nDNA and mtDNA; however, the rate of increase with age was much greater in mtDNA.
Furthermore, a previous study suggested that artifactual oxidation of deoxyguanosine
can occur during sample preparation (33).
The amplification solution consisted of the following: 1 [micro]mol/L of each consensus primer; 200 [micro]mol/L each of deoxyadenosine triphosphate, deoxycytidine triphosphate, and deoxyguanosine
triphosphate; and 140 [micro]mol/L of deoxythymidine triphosphate.
02 mmol/L deoxyadenosine triphosphate, deoxycytosine triphosphate, and deoxyguanosine
triphosphate (Sigma); 0.