Intracellular cytarabine triphosphate production correlates to deoxycytidine
kinase/cytosolic 5'-nucleotidase II expression ratio in primary acute myeloid leukemia cells.
Functional role of alternatively spliced deoxycytidine
kinase in sensitivity to cytarabine of acute myeloid leukemic cells.
To introduce amino functions into the 5'-terminal end of an oligonucleotide during its synthesis for coating and lanthanide-labeling purposes, a diaminohexane-modified deoxycytidine
(modC) (19) was used.
In experiment 4, to demonstrate that the methylation of deoxycytidine
residues was reduced, we performed an enzymatic hydrolysis of the isolated DNA as described below.
phosphoramidite (modC), active lanthanide chelates, streptavidin-coated microtitration plates, DELFIA Assay Buffer, Wash Solution, and Enhancement Solution, and a DELFIA Plateshake, Platewash, Plate Dispense, and 1234 Research Fluorometer were from Wallac Oy (Turku, Finland).
deoxynucleotide triphosphates (dNTPs), which are the nucleotides deoxyadenosine triphosphate (dATP), deoxyguanosine triphosphate (dGTP), deoxycytidine
triphosphate (dCTP), and deoxythymidine triphosphate (dTTP) that compose DNA.
Radu, Nathanson and their colleagues found that an important nucleotide called deoxycytidine
triphosphate (dCTP) is produced by two pathways, the de novo pathway and the nucleoside salvage pathway.
A virtual pyrogram for hypothetical DNA sequence ACGT, where the polymerase chain reaction product bottom-strand with annealed top-strand sequencing primer is shown on the left, shaded box, and the extended nascent strand after addition of deoxyadenosine triphosphate (dATP) and deoxycytidine
triphosphate are shown in the right, shaded box.
FTC, a synthetic nucleoside analog of cytidine, inhibits the activity of HIV reverse transcriptase (RT) by competing with a natural substrate deoxycytidine
5'-triphosphate and by being incorporated into nascent viral DNA resulting in chain termination.
Base Nucleoside Nucleotide Adenine deoxyguanosine deoxyguanylate Guanine deoxyguanosine deoxyguanylate Cytosine deoxycytidine
deoxycytidylate Thymine deoxythymdine deoxythymidylate Table 1.
ABBREVIATIONS: bDNA = Branched DNA; DNA = Deoxyribonucleic Acid; dNTP = Deoxyribonucleotides; dATP = Deoxyadenosine Triphosphate; dCTP = Deoxycytidine
Triphosphate; dGTP = Deoxyguanosine Triphosphate; dTTP = Deoxythymidine Triphosphate; FDA = Food and Drug Administration; FRET = Fluorescence Resonance Energy Transfer; MRSA = Methicillin Resistant Staph aureus; MTB = Mycobacterium tuberculosis; NASBA = Nucleic Acid Sequence Based Amplification; PCR = Polymerase Chain Reaction; PFGE = Pulsed-Field Gel Electrophoresis; RT-PCR = Reverse Transcription Polymerase Chain Reaction; Taq = Thermus aquaticus; TMA = Transcription-Mediated Amplification; VRE = Vancomycin Resistant Enterococcus.
Comparison of the substrate specificities of human thymidine kinase 1 and 2 and deoxycytidine
kinase toward antiviral and cytostatic nucleoside analogs.