Thus, hospital length of stay is much shorter when compared to amphotericin B
deoxycholate regimen, which should be administered for a period of 14-20 days.
Injection lipolysis with a cocktail of phosphatidylcholine and
deoxycholate: an Indian experience.
Wistar rats Regular diet 100 mg/kg in sodium caprate, or sodium
deoxycholate, once i.g.
Amphotericin B
Deoxycholate. Amphotericin B
deoxycholate is also called "conventional" amphotericin B.
To isolate the bacterial colonies, the samples were cultured with the aid of a platinum wire loop on Petri dishes containing blood agar and XLDagar (Xylose Lysine
Deoxycholate agar), using the agar depletion technique.
For bile resistant analysis, fresh culture of KCC-32 was inoculated into the sterilized MRS broth containing bile salts such as 0.5% sodium
deoxycholate and 0.3% oxgall (DCA Sigma, St Louis, MO, USA) and incubated at 37degC.
5% sodium
deoxycholate, 10 mg/ml aprotinin, 10 mg/ml leupeptin, and 1 mM phenylmethylsulfonylfluoride.
For western blot procedures, hypothalamus from AEA-treated and control animals was homogenized (Heidolph homogenizer DIAX 600) in 500 [micro]l of RIPA buffer (25 mM Tris-HCl; pH 7.6; 150 mM NaCl; 1% sodium
deoxycholate; 0.1% SDS) in the presence of protease inhibitor cocktail (Sigma-Aldrich, USA, P2714).
Proteins were extracted from cell pellets using lysis buffer (1 M Tris HCl pH8, Triton X-100 1%, and Na
deoxycholate 0.25%) with the addition of PMSF 1 mM.
The suspension was centrifuged and the pellet was resuspended and washed twice with 50 mM Tris-Cl pH 8.0,300 mM NaCl, 0.25% sodium
deoxycholate, and 5mM DTT (the second time without sodium
deoxycholate).
Treatment was initiated with a daily regimen of amphotericin B
deoxycholate, starting with escalating doses.
A loop full of culture from selenite cysteine broth culture was subcultured onto Xylose lysine
deoxycholate (XLD) agar (Oxoid, England) plate and incubated aerobically at 37[degrees]C for 24 hrs.