The outer layer of the SC consists of a cornified layer made of flattened and denucleated
KCs (or corneocytes) and a SC-specific barrier structure called the cornified envelope that replaces the KC cell membranes.
After 30 minutes of -110 Kpa the polyurethane metacarpal head was pressed up against the inside of a glass beaker wall while completely immersed in denucleated fluid.
It is noteworthy that in the previous model employing nucleated fluid (Model B), bubble formation was observed immediately during traction, whereas in Model C employing denucleated fluid, gas bubble formation was no longer evident early on.
Mature oocytes were denucleated
by aspirating the first polar body and fused with the donor cells enriched in G0 of the cell cycle, as the parameter of two DC pulses of 2.5 kv/cm for 10 ms each at 1 s apart, delivered by a BTX2001 Electro Cell Manipulator (BTX, San Diego, CA, USA).
The donor cell is not injected into the denucleated
oocyte, but is placed in the perivitelline space, whereafter it is combined with the oocyte after electroporation.
Cloning animals using somatic cell nuclear transfer (SCNT), or potentially cloning humans in the future, may also involve aberrant epigenetic programming because the newly formed embryo's first key epigenetic period occurs shortly after fertilization, when the donor nucleus is being integrated into the denucleated
oocyte during SCNT.
Their genetic material was inserted into denucleated
eggs, to produce embryos that grew into four mouse clones.
The pathophysiology of bacterial meningitis includes a process that releases bacterial products such as peptidoglycans, lipoteichoic acid, lipopolysaccharides, and denucleated
(72) This denucleated
ovum and the somatic cell were subjected to an electric stimulus that caused them to fuse.
Then it is removed and fused into another denucleated
egg that has been previously been fertilised by sperm.
A sexual reproduction, accomplished by fusing a denucleated
egg (one with the nucleus, which contains the genetic material, removed) with a cell from the organism being cloned.
When "Dolly," the sheep brought into being by cloning, was announced in February of 1997, it was evident that the time Dolly began was when the nucleus from a somatic cell of Sheep A was fused (electrically) with the denucleated
ovum of Sheep B.