density gradient centrifugation


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den·si·ty gra·di·ent cen·trif·u·ga·tion

ultracentrifugation of substances in concentrated solutions of cesium salts or of sucrose; at equilibrium, the medium exhibits a concentration (hence density) gradient increasing in the direction of centrifugal force and the substances of interest collect in layers at the levels of their densities. See: isopycnic zone.

centrifugation

the process of separating lighter portions of a solution, mixture or suspension from the heavier portions by centrifugal force.

density gradient centrifugation
a procedure for separating particles such as viruses or ribosomes or molecules such as DNA in which the sample is placed on a preformed gradient such as sucrose or cesium chloride. Upon centrifugation either by rate zonal or equilibrium procedures, the macromolecules are 'banded' in the gradient and can be collected as a pure fraction.
References in periodicals archive ?
Comparison of two density gradient centrifugation systems for the enrichment of disseminated tumor cells in blood.
The viabilities of the fresh hemocytes collected from the oysters and the separated hemocyte subpopulations by Percoll discontinuous density gradient centrifugation were estimated by the 0.
Density gradient centrifugation is one of the most used techniques for separating molluscan hemocytes into subpopulations.
However, the density gradient centrifugation with successive Percoll and Ficoll solutions is a practical technique for molluscan hemocyte separation, although it has different efficiency with the different molluscan hemocytes.
Separation of Lymnaea stagnalis hemocytes by density gradient centrifugation.
Separation of Crassostrea gigas hemocytes by density gradient centrifugation and counterflow centrifugal elutriation.
These problems have, in part, been overcome by the introduction of more rapid procedures, including tube gel electrophoresis [Quantimetrix Lipoprint (7)] and nuclear magnetic resonance (8), and wider recognition of a definitive classification of LDL subclasses based on the comparison methods of density gradient centrifugation and GGE (4-6).
Choice of conditions for density gradient centrifugation.