denaturing gradient gel electrophoresis


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denaturing gradient gel electrophoresis (DGGE)

electrophoretic technique for separating DNA molecules on the basis of their sequence. A gradient of increasingly denaturing conditions, using a chemical denaturant such as formamide or urea, is applied across a gel and as double-stranded DNA molecules migrate through the gel, they denature into partially and then fully single-stranded DNA, with a consequent change in electrophoretic mobility, which dictates their final position in the gel.

The stability of the DNA helix to denaturation depends on the G+C content of the molecule. Thus DNA molecules of similar size, but different %G+C will denature and separate at different denaturant concentrations. The general principle of this technique can be applied to separate HETERODUPLEXES from HOMODUPLEXES based on the degree of COMPLEMENTARY BASE PAIRING between the two strands in each molecule.

A variation of this technique, temperature/ thermal gradient gel electrophoresis (TGGE), employs a temperature gradient, rather than chemical denaturants.

References in periodicals archive ?
Effects of fasting on rumen microbiota based on the banding profile of denaturing gradient gel electrophoresis (A, B, and C indicate specific animals).
The detection of Mycoplasma (formerly Eperythrozoon) wenyonii by 16S rDNA PCR and denaturing gradient gel electrophoresis. Vet.
Caption: Figure 1: (a) Denaturing gradient gel electrophoresis (DGGE) fingerprints of 16S rRNA gene fragments amplified from endophytic DNA templates isolated from sweet sorghum plants grown under high (+) and low (-) N fertilization levels (100 and 0 kg N [ha.sup.-1], respectively).
Table 5: Values of R of the analysis of similarity of the culture/ independent total diazotrophic bacterial community determined through Denaturing Gradient Gel Electrophoresis (DGGE) of the nif H gene in different regions of the plant/bacteria association in B.
Denaturing gradient gel electrophoresis analysis to study bacterial community structure in pockets of periodontitis patients.
Denaturing gradient gel electrophoresis (DGGE), one such method, provides a technique that allows relatively small DNA fragments to be separated by single base pair differences (Miller et al.
Details of partial amoA sequences (150 amino acid) of denaturing gradient gel electrophoresis (DGGE) bands and their closest relatives DNA sequences can be accessed through DNA Data Base of Japan (DDBJ: www.ddbj.nig.ac jp) and National Centre for Biotechnology Information (NCBI: www.ncbi.ncbi.nih.gov) Closest relative (closest DGGE band amoA protein sequence) Band Accession Microorganism Accession no.
Denaturing gradient gel electrophoresis of PCR-amplified 16S rDNA--a new molecular approach to analyze the genetic diversity of mixed microbial communities, p.
Denaturing gradient gel electrophoresis (DGGE) is a powerful technique for separation of double-stranded DNA molecules of nearly identical size according to their nucleotide composition.
(11,12) These include fluorescent in situ hybridization, (13-17) denaturing gradient gel electrophoresis, (18,20) and temperature gradient gel electrophoresis.
Identification of five novel inactivating mutation in the human thyroid peroxidase gene by denaturing gradient gel electrophoresis. Hum Murat 6:9-16.
We are currently using nested PCR to amplify MHC loci, and plan to genotype these loci using DGGE (denaturing gradient gel electrophoresis).