denatured protein


Also found in: Dictionary, Thesaurus, Encyclopedia.

de·na·tured pro·tein

a protein whose characteristics or properties have been altered in some way, as by heat, enzyme action, or chemicals, and in so doing has lost its biologic activity.

denatured protein

Etymology: L, de, from, natura, proteios, first rank
a protein that has undergone change that causes its original properties to be lost. A protein can be denatured by radiation, heat, strong acids, or alcohol.

de·na·tured pro·tein

(dē-nāchŭrd prōtēn)
One with characteristics or properties that have been altered in some way, as by heat, enzyme action, or chemicals, and in so doing has lost its biologic activity.

denatured protein

A protein in which the amino acid composition and stereochemical structure (shape) have been altered by physical or chemical means. See: coagulated protein
See also: protein
References in periodicals archive ?
All small heat shock possess chaperone like activity and prevent aggregation of denatured proteins.
We denatured proteins in 3 [micro]L of the plasma sample by adding 6 [micro]L of U9 solution (9 mol/L urea, 20 g/L CHAPS, 50 mmol/L Tris-HCl, pH 9), and diluted them with 51 [micro]L of binding buffer (50 mmol/L sodium acetate, 1 mL/L Triton X-100, pH 4.
Under such environmental conditions Hsps and other molecular chaperones stabilize denaturing proteins, refold reversibly denatured proteins, and facilitate the degradation of irreversibly denatured proteins (Lindquist, 1986; Lindquist and Craig, 1988; Parsell and Lindquist, 1994; Feige et al.
Belman (1969) documented preferential sequestration of topically applied beryllium fluoride in guinea pig epidermis and increased beryllium binding affinity for denatured proteins.
Additionally, the present invention includes a method for the handling of denatured proteins with subsequent renaturation in situ (parenthetically after binding to metal charged iminodiacetic acid cellulose).
It uses gel electrophoresis to separate native or denatured proteins by the length of the polypeptide (denaturing conditions) or by the 3-D structure of the protein (native/ non-denaturing conditions).
Sequential elution of denatured proteins, hydrolyzed RNA, and plasmid DNA of bacterial lysates adsorbed onto stacked DEAE-cellulose membranes.
Denatured proteins can then cause clouding of the contact lenses and, as the body no longer recognises them, can lead to inflammatory responses--the most common one being contact lens papillary conjunctivitis (CLPC).
Inevitably, per gel band may contain several denatured proteins or polypeptides that lead to a mixture of peptides in in-gel digestion.
HSPs are well-characterized and pervasive "chaperones" of nascent peptides during protein synthesis; they also help to re-fold denatured proteins resulting from environmental perturbation of cells (reviewed in Morimoto et al.