denaturation

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denaturation

[de-na″chur-a´shun]

a change in the usual nature of a substance, as by the addition of methanol or acetone to alcohol to render it unfit for drinking, or the change in the physical properties of a substance, such as a protein or nucleic acid, caused by heat or certain chemicals that alter tertiary structure.

protein denaturation any nonproteolytic change in the chemistry, composition, or structure of a native protein that causes it to lose some or all of its unique or specific characteristics.

de·na·tur·a·tion

(dē-na'tyū-rā'shŭn),

The process of becoming denatured.

Farlex Partner Medical Dictionary © Farlex 2012

de·na·tur·a·tion

(dē-nā'chŭr-ā'shŭn)

The process of becoming denatured.

Medical Dictionary for the Health Professions and Nursing © Farlex 2012

denaturation

1. Alteration in the folding pattern of a protein by heat or chemical reaction from its physiological conformation to an inactive shape.

2. In the case of DNA or RNA the conversion from a double-stranded structure to a single stranded structure, usually by heating. This is an essential stage in the POLYMERASE CHAIN REACTION.

Collins Dictionary of Medicine © Robert M. Youngson 2004, 2005

denaturation

the breakdown of the bonds forming the quaternary, tertiary and secondary structures of PROTEINS and NUCLEIC ACIDS, a process that can be caused by various agents, such as excess heat, strong acids or alkalis, organic solvents and ultrasonic vibration. In some cases, denaturation is irreversible as in, for example, the heating of egg albumen to give solid egg white. When denaturation occurs in enzymes, they are inactivated, with major effects in living organisms. Denatured nucleic acids can often be returned to their original configuration in the process of renaturation.

Collins Dictionary of Biology, 3rd ed. © W. G. Hale, V. A. Saunders, J. P. Margham 2005

References in periodicals archive ?

Table 3 also shows the thermal denaturation temperature and enthalpy in normal and PSE-like actomyosin.

Comparative study of thermal gelation properties and molecular forces of actomyosin extracted from normal and pale, soft and exudative-like chicken breast meat

In current study DSC has been employed to study denaturation of [alpha]-casein.

STUDY OF THERMAL AND HYDROLYTIC DENATURATION OF CASEIN ([alpha]S1) USING DIFFERENTIAL SCANNING CALORIMETRY (DSC)

The thermal denaturation of the Hc isoforms [[alpha].sub.D+N]-HaH also was found to be irreversible (Figure 1(b), line C).

Calorimetric Study of Helix aspersa Maxima Hemocyanin Isoforms

Anti-inflammatory activity Compounds % inhibition of % membrane albumin stabilization denaturation (conc.

L-Proline-Catalyzed Synthesis of Phthalimide Derivatives and Evaluation of Their Antioxidant, Anti-Inflammatory, and Lipoxygenase Inhibition Activities

Protein denaturation correlates well with textural characteristics.

Effect of Freezing on the Shelf Life of Salmon

1, in which transient DNA denaturation ("breathing") allows probe binding to fully matched sequence positions and site-specific DNA digestion by DSN.

Molecular Diagnostics and Genetics Multiplexed Elimination of Wild-Type DNA and High-Resolution Melting Prior to Targeted Resequencing of Liquid Biopsies

Strong negative cooperativity (p<<1) shows the irreversible denaturation of HSA by toxic Lanthanide ions.

Lanthanide Chlorides Decrease the Antioxidant Property of Human Serum Albumin

88) The denaturation of what McKibben calls 'natural processes (rain, wind, heat)' is especially apparent in the ways many of us now experience weather.

The denaturation of environmental education: exploring the role of ecotechnologies

Kinetics and thermodynamics for irreversible thermal denaturation of MnP was determined by incubating the enzyme in phosphate buffer (pH 6.0) at different temperatures in the absence of substrate.

Investigation of Catalytic Properties of Manganese Peroxidase (MnP) Produced from Agaricus bisporus A21 and its Potential Application in the Biotransformation of Xenobiotic Compound

The amplification conditions for invA gene consisted of 30 cycles with denaturation at 94[degrees]C for 60 s, annealing at 51[degrees]C for 60 s and extension at 72[degrees]C for 60 s; whereas, typh gene was amplified with 34 cycles with denaturation at 94[degrees]C for 60 s, annealing at 55[degrees]C for 90 s, and extension at 72[degrees]C for 90 s.

Molecular typing of Salmonella Typhimurium and S. Enteritidis serovars from diverse origin by ERIC-PCR

Standard real-time amplification conditions were applied (initial activation step of 50[degrees]C/5 min, denaturation of 95[degrees]C/10 min and 40 cycles of denaturation at 95[degrees]C/30 s and a combined annealing and elongation step at 60[degrees]C for 1 min).

Late emergence of A594V and L595W mutations related to ganciclovir resistance in a patient with HCMV retinitis and long-term HIV progression

The amplification conditions were as follows: denaturation of dsRNA at 95[degrees]C for 5 min, reverse transcription at 45[degrees]C for 45 min, and then amplification of cDNA following the cycling parameters [5].

Prevalence and genotype distribution of rotaviruses in children with gastroenteritis in Rize province