denaturation


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denaturation

 [de-na″chur-a´shun]
a change in the usual nature of a substance, as by the addition of methanol or acetone to alcohol to render it unfit for drinking, or the change in the physical properties of a substance, such as a protein or nucleic acid, caused by heat or certain chemicals that alter tertiary structure.
protein denaturation any nonproteolytic change in the chemistry, composition, or structure of a native protein that causes it to lose some or all of its unique or specific characteristics.

de·na·tur·a·tion

(dē-na'tyū-rā'shŭn),
The process of becoming denatured.

de·na·tur·a·tion

(dē-nā'chŭr-ā'shŭn)
The process of becoming denatured.

denaturation

1. Alteration in the folding pattern of a protein by heat or chemical reaction from its physiological conformation to an inactive shape.
2. In the case of DNA or RNA the conversion from a double-stranded structure to a single stranded structure, usually by heating. This is an essential stage in the POLYMERASE CHAIN REACTION.

denaturation

the breakdown of the bonds forming the quaternary, tertiary and secondary structures of PROTEINS and NUCLEIC ACIDS, a process that can be caused by various agents, such as excess heat, strong acids or alkalis, organic solvents and ultrasonic vibration. In some cases, denaturation is irreversible as in, for example, the heating of egg albumen to give solid egg white. When denaturation occurs in enzymes, they are inactivated, with major effects in living organisms. Denatured nucleic acids can often be returned to their original configuration in the process of renaturation.
References in periodicals archive ?
In current study DSC has been employed to study denaturation of [alpha]-casein.
The thermal denaturation of the Hc isoforms [[alpha].sub.D+N]-HaH also was found to be irreversible (Figure 1(b), line C).
Anti-inflammatory activity Compounds % inhibition of % membrane albumin stabilization denaturation (conc.
Protein denaturation correlates well with textural characteristics.
1, in which transient DNA denaturation ("breathing") allows probe binding to fully matched sequence positions and site-specific DNA digestion by DSN.
Strong negative cooperativity (p<<1) shows the irreversible denaturation of HSA by toxic Lanthanide ions.
88) The denaturation of what McKibben calls 'natural processes (rain, wind, heat)' is especially apparent in the ways many of us now experience weather.
Kinetics and thermodynamics for irreversible thermal denaturation of MnP was determined by incubating the enzyme in phosphate buffer (pH 6.0) at different temperatures in the absence of substrate.
The amplification conditions for invA gene consisted of 30 cycles with denaturation at 94[degrees]C for 60 s, annealing at 51[degrees]C for 60 s and extension at 72[degrees]C for 60 s; whereas, typh gene was amplified with 34 cycles with denaturation at 94[degrees]C for 60 s, annealing at 55[degrees]C for 90 s, and extension at 72[degrees]C for 90 s.
Standard real-time amplification conditions were applied (initial activation step of 50[degrees]C/5 min, denaturation of 95[degrees]C/10 min and 40 cycles of denaturation at 95[degrees]C/30 s and a combined annealing and elongation step at 60[degrees]C for 1 min).
The amplification conditions were as follows: denaturation of dsRNA at 95[degrees]C for 5 min, reverse transcription at 45[degrees]C for 45 min, and then amplification of cDNA following the cycling parameters [5].