demecolcine

demecolcine

 [dem″e-kol´sēn]
a cytotoxic alkaloid derived from Colchicum autumnale. It is used in chromosome analysis to arrest cell division in mid-metaphase so that the chromosomes can be stained by one of several techniques that produce a distinct pattern of light and dark bands along the chromosomes, and each chromosome can be recognized by its size and banding pattern.

dem·e·col·cine

(dem'ĕ-kol'sēn),
An alkaloid from Colchicum autumnale (family Liliaceae) similar chemically to colchicine except that the acetyl group is replaced by a methyl group; used for gout and leukemia, is said to be less toxic than colchicine, and has an action upon mitosis similar to that of colchicine.

demecolcine

a cytotoxic alkaloid derived from Colchicum autumnale. It is used in chromosome analysis to arrest cell division in mid-metaphase so that the chromosomes can be stained by one of several techniques that produce a distinct pattern of light and dark bands along the chromosomes, and each chromosome can be recognized by its size and banding pattern.
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Tenders are invited for Supply of cytochalasin-b from Drechslera dematioidea, use for Cytokinesis blocking, purity, demecolcine, use for blocking mitosis at metaphase, purity>/=98 % powder.
To block the mitotic process of the cells at the metaphase stage, Demecolcine (Sigma-Aldrich) was added for the last 2 h of culture at a final concentration of 10 [micro]g/ml.
This possible difference could be probed by microinjecting a microtubule poison, for example demecolcine, directly into the vegetal ooplasm.
Moreover, three microtubule poisons - colchicine, demecolcine, and nocodazole - inhibit the normal movement of oil droplets toward the vegetal pole, eliminate the saltatory motion of small inclusions, and slow the growth of the blastodisc: eggs treated with [Beta]-lumicolchicine, an inactive derivative of colchicine, segregated normally (Abraham et al.
Working solutions of demecolcine (N-deacetyl-N-methyl-colchicine; Sigma) were prepared by diluting (at least 100X) an aqueous stock solution of 0.
Eggs that were in a solution of demecolcine and being irradiated with UV light were fixed on the stage of the microscope for 5 min before being transferred to a vial of fixative.
Effects of cytochalasin D and demecolcine on ooplasmic segregation and development
The effects of demecolcine on the eggs were those previously described by Abraham et al.
In eggs treated with both CCD and demecolcine, we saw no saltatory movement, no cytoplasmic streaming, and no movement of oil droplets toward the vegetal pole [ILLUSTRATION FOR FIGURE 1D OMITTED].
Reversal of the inhibitory effects of demecolcine on ooplasmic segregation and embryonic development
When demecolcine-treated eggs were irradiated with UV light, the inhibitory effects of demecolcine were reversed: oil droplet movement and saltatory movement were restored; and the eggs subsequently cleaved, formed an embryonic axis, and hatched.
0 [[micro]molar] demecolcine; irradiation with low intensities of UV light), this reversal of the effects of demecolcine on oil droplet movement was restricted to the portion of the egg that was irradiated.