Other subtype B genes that have become pseudogenes in subtype A specimens and are associated with metabolic pathways are those encoding a haloacid dehalogenase
(LBJ_RS17075) and a methylmalonyl-CoA mutase (LBJ_RS11290), although these genes appear to have putative paralogs with similar function in the genome.
In this work, the bacterial proteins of interest were tagged with a HaloTag, a modified haloalkane dehalogenase
that covalently binds to synthetic Halo-ligands (haloalkanes) that can be fluorescently labeled, thus allowing more robust detection of bait protein binding to interactors present on the array.
In the paper titled "Identification of Multiple Dehalogenase
Genes Involved in Tetrachloroethene-to-Ethene Dechlorination in a Dehalococcoides-Dominated Enrichment Culture," M.
(1.) Anandaraj a K, Kiefer PM, Donhoe JB S, Copley SD, Recruitment of a double bond isomerase to serve as a reductive dehalogenase
during biodegradation of pentachlorophenol.
D gene (dehD) capable of degrading monochloroacetic acid (MCA) has been previously isolated from Rhizobium sp.
Interestingly, certain members of the class Flavobacteria, which includes Flavobacterium spp., were reported to possess dehalogenase
activity, and although it has not been demonstrated in isolates derived from the mammalian gut, they may contribute to the metabolism of TCDF and other halogenated compounds (Xun et al.
melanogaster by a FPP phosphatase (FPPase or FPPP) , a member of the NagD halo alkanoic acid dehalogenase
family (HAD), with orthologues in several insect species, including A.
The theta class of GSTs is represented by dichloromethane dehalogenase
produced by methylotrophic bacteria , while the zeta class is represented by tetrachlorohydroquinone (TCHQ) dehalogenase
Monooxygenase type dehalogenase
adds one atom of oxygen to chlorinated compounds to remove the chlorine atom .
Quantitative PCR targeting 16s rRNA and reductive dehalogenase
genes simultaneously monitors multiple dehalococcoides strains.