When gram-negative bacteria are rinsed with a decolorizer
such as acetone, the lipids in their cell walls are removed resulting in open pores.
The additional elements can provide more points of comparison, potentially improving discrimination capability, and can be used to detect specific elements present in some products, such as Ce when it is added as a decolorizer
It can be demonstrated in direct smears by modified Kinyoun stain, in which acid-alcohol decolorizer
is substituted with a low-concentration inorganic acid.
In a second group of males, I reduced badge size using a hair decolorizer
applied on the edges of the throat patch (badge-reduced males).
The strain B5 was found to be the least efficient decolorizer
of the dye among the tested strains.