The cochleae from Myo3a mutant and wild-type mice were removed, fixed with 2.5% glutaraldehyde in 0.1 M PBS at 4[degrees]C overnight, and decalcified
in 10% EDTA .
Examples include questions regarding decalcified
tissues and revalidation processes upon change of antibody clone.
The results mentioned above, obtained by using cutting sections of decalcified
tissue or separated auditory ossicles, are rather imprecise compared with today's technological possibilities.
samples were completely immersed in this solution and incubated at room temperature for 24 (grafts) or 48 hours (bones).
This study utilized consecutive sections prepared for either decalcified
tissue testing after paraffin embedding or undecalcified tissue investigating after PMMA embedding.
Urist, "Bone induction in excavation chambers in matrix of decalcified
dentin," Archives of Surgery, vol.
We assessed the location of the phosphoproteins within the barnacle glue layer using decalcified
barnacle base plates and residual glue deposited by reattached barnacles.
Biopsy fragments were kept in formaline for 24 hours and decalcified
in 3% nitric acid.
The mass including the bone was decalcified
with K-CX (Falma Corporation, Tokyo, Japan).
Additionally, after fixation, bone tissue specimens taken from inferior metaphysis of the right femur were decalcified
. Five-micron tissue sections were examined under routine--hematoxylineosine (HE) and trichromic--staining.
Following DEXA measurements of the distraction gaps, specimes were fixed in 10% buffered paraformaldehyde for 48 hours and decalcified
in ethylenediaminetetraacetic acid (EDTA) solution.
The treatment has its greatest effect when applied before the onset of caries, but it also may help halt or reverse early carious lesions, which present as decalcified
"white spot lesions" along the gum line.