Nevertheless, at p4, the deamidation
rate was increased after EPX treatment.
RP-HPLC-UV/MS peptide mapping was used to detect posttranslational modifications, such as deamidation
, oxidation, and phosphorylation.
It is also utilized in facilitating some chemical reaction like deamidation
, rearrangement, cyclization and imidation .
A stress protein on their surface is presented, permeability changes results in the entry of gliadin in laminapropria, where its deamidation
by an enzyme tissue transglutaminase, allowing its interaction with HLA-DQ2 (or HLA-DQ8) on antigen presenting cell's surface.11 Presentation of gliadin to reactive CD4+T cell via T-cell receptor, causes the cytokine release with resulting tissue damage and expansionof B cells that produce antibodies.22
Tissue transglutaminase plays an important role as it crosslinks gliadin and cause its deamidation
into glutamic acid and gliadin peptide.
of collagen makes it a useful and important biopolymer in the field of bio-material engineering.
Less critical were truncated variants, norleucine and deamidation
, all of which were assessed using some form of chromatography.
of asparagine and glutamine and oxidation of methionine were selected for the search parameters as variable modification, and carbamidomethyl modification of cysteine was used as a fixed modification.
is considered as the most common route for chemical degradation of proteins, peptides and antibodies (15,16).
Settings were as follows: instrument type set as FT-ICR/Orbitrap; high energy CID as fragmentation mode; parent ion error tolerance 8 ppm; fragment ion error tolerance 0.03 Da; trypsin as enzyme; up to one missed cleavage allowed; carbamidomethylation as a fixed modification; deamidation
and oxidation as variable modifications.
The six Asn residues in hIAPP render the molecule susceptible to deamidation
. Spontaneous Asn deamidation
is one of the most common nonenzymatic posttranslation modifications of proteins and is believed to play a role in amyloid formation by other polypeptides .
From a single digest and run, users can gain identity and purity information with 100% coverage in a peptide map; heterogeneity information with ultra-sensitive glycopeptide quantification; and stability information identifying, deamidation
, cyclization and oxidation.